Catalog # DP3491

mDia2 (C-terminal region) Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 135 kDa

Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. The FH1 region contains poly-proline stretches that promote interactions with profilin. The FH2 domain, located C-terminally to the FH1 domain, is highly conserved in formin proteins and possesses actin nucleation and polymerization activities. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. A subgroup of the formins is the diaphanous (Dia) family, which includes mDia1 (Diap1), mDia2 (Diap3), and mDia3 (Diap2). The mDia2 protein has been implicated in cell migration and cytokinesis. This Dia protein can nucleate actin polymerization, as well as bind and stabilize microtubules. mDia2 may also have functions in the nucleus, since it is continually shuttled between the cytoplasm and nucleus.

References

Miki, T. et al. (2009) J Biol Chem. 284:5753.
Bartolini, F. et al. (2008) J Cell Biol. 181:523.
Alberts, A.S. (2001) J Biol Chem. 276:2824.

Western blot analysis of mDia2 expression in rat PC12 (lane 1), human A431 (lane 2), mouse brain (lane 3), and rabbit spleen fibroblasts (lane 4). The blots were probed with anti-mDia2 (C-terminal region) at 1:500.

mDia2 synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of mouse mDia2 (Diap3). This peptide sequence is highly conserved in rat and human mDia2, and has low homology to other formins.

*For more information, see UniProt Accession Q9Z207

Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using mDia2 (C-terminal region) peptide (without carrier). The antibody detects a 135 kDa* protein corresponding to the apparent molecular mass of mDia2 on SDS-PAGE immunoblots of various cells and tissues, including human A431 and HeLa cells, mouse brain tissue and C2C12 cells, rat PC12 cells, and rabbit spleen fibroblasts.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Egami, Y. et al. (2017). J Cell Sci. 130(24):4168. (WB: mouse macrophage cells)
Li X. et al. (2017) Haematologica. 102(6):984. (ICC: fetal liver cells)
Spence, EF et al. (2016) J Neurosci. 36(37):9696. (WB: mouse postnatal brain)
Monzo, P. et al. (2016) Mol Biol Cell. 27(8):1246. (WB: rat C6 glioma, shRNA knockdown)
Belin, BJ et al. (2015) Elife. Aug 19;4:e07735. (WB: human HeLa, shRNA knockdown)
Tabdanov, E. et al. (2015) Integrative Biology 7(10):1272 (ICC: human Jurkat T cells)
Sandbo, N. et al. (2013) J Biol Chem 288(22):15466. (WB : human lung fibroblasts)

This kit contains: