Catalog # CM5131
p21/CIP1/WAF1 Antibody
Mouse Monoclonal
| ICC | 1:50 |
| IHC | 1:50 |
| IP | 1:50 |
| WB | 1:500 |
Size 100 μl
Species Reactivity Hu
MW 21 kDa
Isotype IgG2a
The tumor suppressor protein p21/CIP1/WAF1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S. However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D. The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression. Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element. Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation.
References
Cheng, J. et al. (1999) EMBO J. 18:1571.
Flores-Rozas, H. et al. (1994) PNAS, USA. 91:8655.
Western blot analysis of p21 expression in human endothelial cells (lanes 1 & 2). The blots were probed with mouse monoclonal anti-p21 at 1:250 (lane 1) and 1:1000 (lane 2).
*For more information, see UniProt Accession P38936
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
This kit contains:
