Catalog # MP4551

Myosin 7B/MHC14 (Hinge region) Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
ICC	1:300
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 215 kDa

Myosin is a highly conserved, ubiquitous protein found in all eukaryotic cells, where it provides the motor function for diverse movements such as cytokinesis, phagocytosis, and muscle contraction. All myosins contain an amino-terminal motor/head domain and a carboxy-terminal tail domain. The class II myosins, consist of the conventional two-headed myosins that form filaments and are composed of two myosin heavy chain (MYH) subunits and four myosin light chain subunits. There are 15 MYH genes identified as Class II myosins and these include six skeletal muscle MYHs (MYH1, MYH2, MYH3, MYH4, MYH8, MYH13), three cardiac MYHs (MYH6, MYH7, MYH7B) and two non-muscle MYHs (MYH9, MYH10), as well as a smooth muscle MYH (MYH11).

References

Rubio, M.D., et al. (2011) J Neurosci. 31(4):1448.
Rossi, A.C. et al. (2009) J Physiol. 588(Pt 2):353.
Berg, J.S. et al. (2001) Mol Biol Cell. 12:780.

Western blot analysis MYH7B in mouse brain (lane 1 & 2) and mouse extraocular muscle (lane 3 & 4). The blot was probed with rabbit polyclonal anti-MYH7B/MHC14 (Hinge region) at 1:500 in absence (lanes 1 & 3) and presence of MYH7B blocking peptide (MX4555; lanes 2 & 4).

Immunocytochemical labeling of MYH7B in rat PC12 cells differentiated with NGF. The cells were probed with MYH7B (Hinge region) rabbit polyclonal antibody (MP4551) in the absence (left) or presence (right) of blocking peptide (MX4555). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the hinge region from mouse MYH7B/MHC14. This sequence is highly conserved in rat and human MYH7B, and has less than 50% identity with other MYH family members.

*For more information, see UniProt Accession A2AQP0

Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using MYH7B (Hinge region) peptide (without carrier). The antibody detects a 215 kDa* protein corresponding to the apparent molecular mass of MYH7B on SDS-PAGE immunoblots of mouse extraocular muscle and brain.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: