Catalog # JP3991

JMY (C-terminal region) Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
ICC	1:200
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck, Fr

MW 130 kDa

JMY (junction mediating and regulatory protein) is a transcription co-factor, originally identified as a p300-binding protein involved in p53-dependent transcription. Upon DNA damage, JMY is released from Mdm2 inhibition and forms a complex with Strap and p300. This complex recruits PRMT5 to activate the p53 response. Through regulation of p53-dependent transcription, JMY has important roles in the DNA damage response. In addition, JMY contains three carboxyl-terminal WH2 actin binding domains which are are commonly found in WASP family proteins. JMY can bind to actin and to the Arp2/3 complex, as well as direct the assembly of actin filaments in vitro. These actin-regulating effects of JMY may have important roles in cell migration. When slow migrating HL-60 cells are differentiated into highly motile neutrophil-like cells, JMY moves from the nucleus to the cytoplasm and is concentrated at the actin-rich leading edge of cells. The loss of JMY leads to decreased cell migration in HL-60 cells. Thus, JMY represents a new class of multifunctional actin assembly factors whose activity may be regulated by cellular localization.

References

Coutts, A.S. et al. (2009) PNAS 106(47):19872.
Zuchero, J.B. et al. (2009) Nat Cell Biol. 11(4):451.
Shikama, N. et al. (1999) Molecular Cell 4:365.

Western blot showing JMY expression in rat PC12 cells (lane 1), human Jurkat cells (lane 2), and adult mouse heart (lane 3). The blots were probed with anti-JMY (C-terminal region) rabbit polyclonal antibody at 1:500.

Immunocytochemical labeling of JMY relative to F-actin in chick fibroblasts. The cells were labeled with rabbit polyclonal JMY antibody (JP3991), then detected using appropriate secondary antibody (Green). This labeling is compared to F-actin staining (Red). (Image provided by Dr. Gianluca Gallo at Drexel University).

JMY synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of mouse JMY. This sequence is highly conserved in human, rat, frog, and zebrafish JMY proteins, and has low homology to other WASP family members.

*For more information, see UniProt Accession Q9QXM1

Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using JMY (C-terminal region) peptide (without carrier). The antibody detects a 130 kDa* protein corresponding to the molecular mass of JMY on SDS-PAGE immunoblots of human HeLa, Jurkat cells, rat PC12 cells, and mouse brain and heart.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: