Catalog # WK6130
WAVE Phospho-Regulation
Antibody Sampler Kit
The Wiskott–Aldrich syndrome protein (WASP) family is involved in various pathways that regulate actin cytoskeletal organization. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms, WAVEs 1, 2, and 3. WAVE proteins play key roles in actin-mediated cell events, such as membrane ruffling and lamellipodia formation. WAVEs contain an N-terminal WAVE homology domain, a basic domain, a Proline-rich region, and carboxy terminal verprolin, cofilin, and acidic (VCA) region. WAVEs are thought to act downstream of the Rac GTPase, connecting Rac activation to induction of Arp 2/3-mediated actin polymerization. Regulation of WAVE activity can occur through tyrosine phosphorylation. Src phosphorylation of WAVE1 at Tyr-125 enhances binding to the Arp2/3 complex, and is required for WAVE inhibition of Arp2/3-mediated stress fiber formation. By contrast, WAVE2 phosphorylation of Tyr-150 by Abl may enhance Arp2/3 complex actin nucleation and microspike formation in fibroblasts. Thus, site-specific tyrosine phosphorylation may be important for controlling specific activities of WAVE proteins.
References
Suetsugu, S. et al. (1999) Bioch. Biophs. Res. Comm. 260:296.
Leng, Y. et al. (2005) PNAS 102(4):1098.
Ardern, H. et al. (2006) Cell Motil. Cytosk. 63:6.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblastsRS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
WP1731 Spillane, M. et al. (2011) Dev Neurobiol. 71(9):747. ICC: chick dorsal root ganglia
WP1821 Bae, H.B. et al. (2011) FASEB J. 25(12):4358. WB: mouse peritoneal macrophages
Western blot of human SYF cSrc transformed cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with pervanadate (1 mM; 30 min). The blots were probed with anti-WAVE1 (N-terminal region) (lanes 1 & 2) or anti-WAVE (Tyr-125) (lanes 3 & 4).
Western blot of human SYF cSrc-transformed cells. Blots were were probed with anti-WAVE1 (N-terminal region) at a dilution of 1:1000 (lane 1), 1:2000 (lane 2) or 1:4000 (lane 3). In addition, the antibody was used in the absence (lane 4) or presence of blocking peptides, WAVE1 (N-terminal region) peptide (lane 5) or WAVE2 (Central region) peptide (lane 6).
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| WAVE1 (N-terminal region) Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 80 | |
| WAVE1 (Tyr-125), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 78 | |
| WAVE2 (Central region) Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 78 | |
| WAVE2 (Tyr-150)[conserved site], phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 78 | |
| Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb | 100 μl | WB, E, ICC, IHC | Rb |
KIT SUMMARY
The WAVE phospho-regulation antibody sampler kit can be used to examine phosphorylation of WAVE1 at Tyr-125 and Tyr-150, as well as the conserved sites in WAVE2 and WAVE3. The kit also includes polyclonal antibodies to monitor total expression levels of WAVE1 and WAVE2.
