Catalog # WK6130

WAVE Phospho-Regulation Antibody Kit

Antibody Sampler Kit



The Wiskott–Aldrich syndrome protein (WASP) family is involved in various pathways that regulate actin cytoskeletal organization. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms, WAVEs 1, 2, and 3. WAVE proteins play key roles in actin-mediated cell events, such as membrane ruffling and lamellipodia formation. WAVEs contain an N-terminal WAVE homology domain, a basic domain, a Proline-rich region, and carboxy terminal verprolin, cofilin, and acidic (VCA) region. WAVEs are thought to act downstream of the Rac GTPase, connecting Rac activation to induction of Arp 2/3-mediated actin polymerization. Regulation of WAVE activity can occur through tyrosine phosphorylation. Src phosphorylation of WAVE1 at Tyr-125 enhances binding to the Arp2/3 complex, and is required for WAVE inhibition of Arp2/3-mediated stress fiber formation. By contrast, WAVE2 phosphorylation of Tyr-150 by Abl may enhance Arp2/3 complex actin nucleation and microspike formation in fibroblasts. Thus, site-specific tyrosine phosphorylation may be important for controlling specific activities of WAVE proteins.

Miki, H. et al. (1999) J Biol. Chem. 274(39):27605.
Suetsugu, S. et al. (1999) Bioch. Biophs. Res. Comm. 260:296.
Leng, Y. et al. (2005) PNAS 102(4):1098.
Ardern, H. et al. (2006) Cell Motil. Cytosk. 63:6.

Western blot of human SYF cSrc transformed cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with pervanadate (1 mM; 30 min). The blots were probed with anti-WAVE1 (N-terminal region) (lanes 1 & 2) or anti-WAVE (Tyr-125) (lanes 3 & 4).

Western blot of human SYF cSrc-transformed cells. Blots were were probed with anti-WAVE1 (N-terminal region) at a dilution of 1:1000 (lane 1), 1:2000 (lane 2) or 1:4000 (lane 3). In addition, the antibody was used in the absence (lane 4) or presence of blocking peptides, WAVE1 (N-terminal region) peptide (lane 5) or WAVE2 (Central region) peptide (lane 6).

Rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
WP1731 Spillane, M. et al. (2011) Dev Neurobiol. 71(9):747. ICC: chick dorsal root ganglia
WP1821 Bae, H.B. et al. (2011) FASEB J. 25(12):4358. WB: mouse peritoneal macrophages

This kit contains:

WAVE1 (N-terminal region) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms80
WAVE1 (Tyr-125), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms78
WAVE2 (Central region) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms78
WAVE2 (Tyr-150)[conserved site], phospho-specific Rabbit pAb 50 μlWB, E, ICCHu, Rt, Ms78
Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb100 μlWB, E, ICC, IHCRb

The WAVE phospho-regulation antibody sampler kit can be used to examine phosphorylation of WAVE1 at Tyr-125 and Tyr-150, as well as the conserved sites in WAVE2 and WAVE3. The kit also includes polyclonal antibodies to monitor total expression levels of WAVE1 and WAVE2.