Catalog # WK6130
WAVE Phospho-Regulation Antibody Kit
Antibody Sampler Kit
| ELISA, WB, ICC, IHC |
Species Reactivity
| Hu, Rt, Ms, Rb |
 |
KIT SUMMARY The WAVE phospho-regulation antibody sampler kit can be used to examine phosphorylation of WAVE1 at Tyr-125 and Tyr-150, as well as the conserved sites in WAVE2 and WAVE3. The kit also includes polyclonal antibodies to monitor total expression levels of WAVE1 and WAVE2. |
Western blot of human SYF cSrc transformed cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with pervanadate (1 mM; 30 min). The blots were probed with anti-WAVE1 (N-terminal region) (lanes 1 & 2) or anti-WAVE (Tyr-125) (lanes 3 & 4).
Western blot of human SYF cSrc-transformed cells. Blots were were probed with anti-WAVE1 (N-terminal region) at a dilution of 1:1000 (lane 1), 1:2000 (lane 2) or 1:4000 (lane 3). In addition, the antibody was used in the absence (lane 4) or presence of blocking peptides, WAVE1 (N-terminal region) peptide (lane 5) or WAVE2 (Central region) peptide (lane 6).
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblastsRS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
WP1731 Spillane, M. et al. (2011) Dev Neurobiol. 71(9):747. ICC: chick dorsal root ganglia
WP1821 Bae, H.B. et al. (2011) FASEB J. 25(12):4358. WB: mouse peritoneal macrophages
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| WAVE1 (N-terminal region) Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 80 | |
| WAVE1 (Tyr-125), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 78 | |
| WAVE2 (Central region) Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 78 | |
| WAVE2 (Tyr-150)[conserved site], phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 78 | |
| Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb | 100 μl | WB, E, ICC, IHC | Rb |
