Catalog # EK6960
EB3 Phospho-Regulation Antibody Kit
Antibody Sampler Kit
| ELISA, WB, ICC |
Species Reactivity
| Hu, Rt, Ms |
 |
KIT SUMMARY The end-binding 3 (EB3) phospho-regulation antibody sampler kit can be used to detect the phosphorylation of Ser-162 and Ser-176 in EB3. The kit includes phospho-specific antibodies, as well as rabbit polyclonal and rat monoclonal antibodies for detecting total EB3 expression. |
Western blot analysis of adult mouse brain untreated (lanes 1, 3, 5, & 7) or treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with rat monoclonal anti-EB3 (EM5091) (lanes 1 & 2), and rabbit polyclonals anti-EB3 (Ser-162) (lanes 3 & 4), anti-EB3 (Ser-176) (lanes 5 & 6), and anti-EB3 (a.a. 171-182) (lanes 7 & 8).
Immunocytochemical labeling of EB3 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-α-Tubulin (TM4111) (green) and rat monoclonal anti-EB3 (EM5091) (red). The antibodies were detected using either goat anti-mouse:DyLight® 488 (MS3011) or goat anti-Rat:DyLight® 594 (RS3111).
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
EM5101 Komarova, Y. et al. (2005) Mol Biol Cell. 16(11):5334. WB, ICC: CHO-K1 celllsThis kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| EB3 Rat mAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 32 | |
| EB3 Rat mAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 32 | |
| EB3 (a.a. 171-182) Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 32 | |
| EB3 (Ser-162), phospho-specific Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 32 | |
| EB3 (Ser-176), phospho-specific Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 32 |
