Catalog # MK6490

Myosin Light Chain Phospho-Regulation Antibody Kit

Antibody Sampler Kit

Application / Dilution
ELISA, WB, ICC, IHC

Species Reactivity

Hu, Rt, Ms, Rb, Ck, Cw

$395


DATASHEET  

KIT SUMMARY

The myosin regulatory light chain (MLC) phospho-regulation antibody sampler kit can be used to examine phosphorylation of MLC at Ser-1 and Ser-19. The kit also includes a polyclonal antibody to monitor total expression levels of MLC, as well as secondary reagent for antibody detection.

Western blot analysis of C2C12 cells untreated (lanes 1, 3, 5, & 7) or treated with Lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with monoclonal anti-MLC20 (clone MY-21) (lanes 1 & 2), polyclonal anti-MLC (Ser-19) phosho-specific (lanes 3 & 4), anti-MLC (Ser-1) phosho-specific (lanes 5 & 6), or anti-MLC (a.a. 11-22) (lanes 7 & 8).

Immunocytochemical labeling of phosphorylated MLC in paraformaldehyde fixed A7r5 cells. The cells were dual-labeled with anti-MLC (MM3441; middle) and anti-MLC (MP4201; top left), anti-MLC (Ser-19) (MP4221; middle left) and anti-MLC (Ser-1) (MP3461; bottom left). Goat anti-Mouse DyLight® 488 and Goat anti-Rabbit DyLight® 594 were used for detection of primary antibodies. The overlay of staining patterns are shown to the right.



Rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagent is supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

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*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

MP4221 Ho, H. et al. (2013) Pigment Cell Melanoma Res 26(2):218. WB: human A375 melanoma
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
MP3461 Aguilar, H.N. et al. (2011) PLoS ONE 6(6):e20903. WB: human uterine myocytes
MP3461 Beach, J.R. et al. (2011) BMC Cell Biol. 12:52. ICC: PHK and HeLa cells
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
Myosin Light Chain (a.a. 11-22) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck20
Myosin Light Chain (Ser-1), phospho-specific Rabbit pAb 50 μlWB, E, ICCHu, Rt, Ms, Cw20
Myosin Light Chain (Ser-19), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck20
Anti-Rabbit Ig Light ChainSpecific:HRP Mouse mAb 100 μlWB, E, ICC, IHCRb