Catalog # WK6110

N-WASP Phospho-Regulation Antibody Kit

Antibody Sampler Kit

Application / Dilution
ELISA, WB, ICC

Species Reactivity

Hu, Rt, Ms

$395


DATASHEET  

KIT SUMMARY

The N-WASP phospho-regulation antibody sampler kit can be used to examine phosphorylation of N-WASP at Tyr-256 and Ser-484/Ser-485. For the latter site, phosphorylated relative to unphosphorylated Ser-484/Ser-485 can be monitored. In addition, the conserved sites in WASP (Tyr-291 and Ser-483/Ser-484) can also be examined. The kit includes polyclonal antibodies to monitor total expression levels of N-WASP and WASP.

Western blot analysis of control and alkaline phosphatase-treated (AP) neonatal rat brain lysate (20 μg/lane). Blots were probed with anti-N-WASP (Lanes 1 & 2), anti-phospho-N-WASP (S484/S485) (Lanes 3 & 4), or anti-unphosphorylated-N-WASP (S484/S485) (Lanes 5 & 6).

Immunocytochemical labeling of N-WASP in control and pervanadate-treated A431 cells. The cells were labeled with rabbit polyclonal N-WASP/WASP (WP2101) or rabbit polyclonal N-WASP (Tyr-256) (WP2601) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight 594.).



Rabbit polyclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

WP2601 Uenishi, E. et al. (2013) J of Bio Chem 288(36): 25851. WB: MIN6-K8 β-cells
WP2601 Cheney, L. et al. (2011) J Infectious Diseases. 203:1824. WB: mouse adipocytes
WP2101 Cheney, L. et al. (2011) J Infectious Diseases. 203:1824. WB: mouse adipocytes
WP2201 Cheney, L. et al. (2011) J Infectious Diseases. 203:1824. WB: mouse adipocytes
WP2001 Anitei, M. et al. (2010) Nature Cell Biol. 12:330. WB: human HeLa cells
WP2601 Pichot, C.S. et al. (2009) British J Cancer. 1 –10. WB: breast cancer cell lines
WP2001 Hartig, S.M. et al. (2009) J Cell Sci. 122:2283. WB: rat L6 myoblasts
WP2001 Pichot, C.S. et al. (2009) British J Cancer. 1 –10. WB: breast cancer cell lines

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
WASP / N-WASP Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms63
N-WASP Rabbit pAb50 μlWB, EHu, Rt, Ms65
N-WASP (Tyr-256), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms65
N-WASP (Ser-484/Ser-485), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms65
unphosphorylated N-WASP (Ser-484/Ser-485) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms65