Vascular endothelial growth factor receptor-2 (VEGFR-2/Flk-1/KDR) is the primary receptor for VEGF in endothelial cells. Other VEGFR family members, VEGFR-1 (Flt-1) and VEGFR-3 (Flt-4), can also transduce the intracellular signals of VEGF. However, the role of VEGFR-1 is observed mainly during embryonic angiogenesis and VEGFR-3 signaling may be restricted to specific types of endothelial cells. Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr-951/996) and in the tyrosine kinase catalytic domain (Tyr-1054/1059). Other sites, Tyr-1175 and Tyr-1212 provide docking sites for downstream signaling molecules. Activation of VEGFR-2 also phosphorylates Tyr-801, leading to PI3-kinase-Akt activation and increases in endothelial nitric oxide synthase activity. Phosphorylation of mutliple sites in VEGFR-2 is required for downstream activation of several signaling pathways that control proliferation, chemotaxis, and sprouting during angiogenesis.
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Kiselyov, A. et al. (2007) Expert Opin. Investig. Drugs 16(1):83.
Garcia Blanes, M. et al. (2007) J Biol. Chem. 282(14):10660.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:VP2921 Chen, P. et al. (2014) Proc Natl Acad Sci. 111(15):5514 WB: HUVECs
VP2921 Chen, T.T. et al. (2010) J Cell Biol. 188(4):595. WB: HUVECs + VEGF
This kit contains:
|CATALOG#||DESCRIPTION||SIZE||APPLICATIONS||SPECIES REACTIVITY||MW (kDa)|
|VEGFR-2 (a.a. 1304-1317) Rabbit pAb||50 μl||WB, E, ICC||Hu, Rt, Ms||180|
|VEGFR-2 (Tyr-801)[conserved site], phospho-specific Rabbit pAb||50 μl||WB, E, ICC||Hu, Rt, Ms||180|
|VEGFR-2 (a.a. 1304-1317) Peptide||50 μg||AB, E|
|phospho-VEGFR-2 (Tyr-801) Peptide||50 μg||AB, E|
|VEGFR-3 (a.a. 1285-1298) Rabbit pAb||50 μl||WB, E||Hu, Rt||170|
|VEGFR-3 (a.a. 1285-1298) Peptide||50 μg||AB, E|
The VEGFR phospho-regulation antibody sampler kit can be used to detect the level of phosphorylation of VEGFR-2 (Tyr-801) relative to the total expression level of VEGFR-2. In addition, the anti-VEGFR-2 (Tyr-801) can be used to detect phosphorylation of the conserved sites in VEGFR-1 (Tyr-794) and VEGFR-3 (Tyr-812). The kit also includes a VEGFR-3 antibody, as well as peptides for antibody blocking experiments.