Catalog # VK6090

VEGFR Phospho-Regulation Antibody Kit

Antibody Sampler Kit



Vascular endothelial growth factor receptor-2 (VEGFR-2/Flk-1/KDR) is the primary receptor for VEGF in endothelial cells. Other VEGFR family members, VEGFR-1 (Flt-1) and VEGFR-3 (Flt-4), can also transduce the intracellular signals of VEGF. However, the role of VEGFR-1 is observed mainly during embryonic angiogenesis and VEGFR-3 signaling may be restricted to specific types of endothelial cells. Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr-951/996) and in the tyrosine kinase catalytic domain (Tyr-1054/1059). Other sites, Tyr-1175 and Tyr-1212 provide docking sites for downstream signaling molecules. Activation of VEGFR-2 also phosphorylates Tyr-801, leading to PI3-kinase-Akt activation and increases in endothelial nitric oxide synthase activity. Phosphorylation of mutliple sites in VEGFR-2 is required for downstream activation of several signaling pathways that control proliferation, chemotaxis, and sprouting during angiogenesis.

Meyer, M. et al. (1999) EMBO J. 18:363.
Robinson, C.J. & Stringer, S.E. (2001) J. Cell Sci.114:853.
Kiselyov, A. et al. (2007) Expert Opin. Investig. Drugs 16(1):83.
Garcia Blanes, M. et al. (2007) J Biol. Chem. 282(14):10660.

Western blot image of GST-recombinant human VEGFR-1 (89 kDa), VEGFR-2 (110 kDa), and VEGFR-3 (86 kDa) C-terminal regions. The blots were probed with rabbit polyclonal anti-VEGFR-2 (a.a. 1304-1317), anti-VEGFR-2 (Tyr-801), and anti-VEGFR-3 (a.a. 1285-1298).

Immunocytochemical labeling of VEGFR-2 in pervanadate-treated HUVEC cells. The cells were labeled with rabbit polyclonal VEGFR-2 (a.a. 1304-1317) and VEGFR-2 (Tyr-801) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. Phase images are shown on the Left.

Rabbit polyclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The peptides are supplied in PBS and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

VP2921 Chen, P. et al. (2014) Proc Natl Acad Sci. 111(15):5514 WB: HUVECs
VP2921 Chen, T.T. et al. (2010) J Cell Biol. 188(4):595. WB: HUVECs + VEGF

This kit contains:

VEGFR-2 (a.a. 1304-1317) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms180
VEGFR-2 (Tyr-801)[conserved site], phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms180
VEGFR-2 (a.a. 1304-1317) Peptide50 μgAB, E
phospho-VEGFR-2 (Tyr-801) Peptide50 μg AB, E
VEGFR-3 (a.a. 1285-1298) Rabbit pAb50 μlWB, EHu, Rt170
VEGFR-3 (a.a. 1285-1298) Peptide 50 μgAB, E

The VEGFR phospho-regulation antibody sampler kit can be used to detect the level of phosphorylation of VEGFR-2 (Tyr-801) relative to the total expression level of VEGFR-2. In addition, the anti-VEGFR-2 (Tyr-801) can be used to detect phosphorylation of the conserved sites in VEGFR-1 (Tyr-794) and VEGFR-3 (Tyr-812). The kit also includes a VEGFR-3 antibody, as well as peptides for antibody blocking experiments.