Catalog # VK6020

Vav Phospho-Regulation Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395

The Vav family of Rho-guanine nucleotide exchange factors, Vav1, Vav2, and Vav3, have central roles in transducing signals from cell surface receptors, such as integrin, growth factor and immune cell receptors to the cytoskeleton. This role includes receptor-mediated changes in the actin cytoskeleton and cell motility. Vav1 expression is normally restricted to hematopoietic cells, while Vav2 and Vav3 are more widely expressed. All three Vav isoforms have been shown to be abnormally expressed in several types of cancer. Vavs are composed of multiple domains including a Dbl homology domain, a calponin homology domain, an acidic amino acid region, a pleckstrin homology domain, a cysteine-rich domain, and SH3 and SH2 domains. Vav activity is regulated by the phosphorylation status of several conserved tyrosine residues in the acidic region (In Vav2: Tyr-142, Tyr-159, and Tyr-172). These tyrosine residues are able to participate in autoinhibitory interactions with the Dbl homology domain and this interaction is prevented after phosphorylation of these sites leading to activation of Vav GEF activity.

References
Schuebel, K.E. et al. (1996) Oncogene 13:363.
Aghazadeh, B. (2000) Cell 102:625.
Wilsbacher, J.L. et al. (2006) Cell Comm. Signal. 4:5.

Western blot of human A431 cells treated with EGF (lanes 1, 3, & 5) then the blot was exposed to alkaline phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Vav (a.a. 165-174) (lanes 1 & 2), anti-Vav2 (Tyr-142) (lanes 3 & 4), or anti-Vav2 (Tyr-172) (lanes 5 & 6).

Western blot of human Jurkat (lanes 1 & 4), HUVEC (lanes 2 & 5), and A431 (lanes 3 & 6) cells. The blots were probed with anti-Vav2 (a.a. 309-322) at a dilution of 1:500 (lanes 1-3) and anti-Vav3 (a.a. 293-305) at 1:500 (lanes 4-6).



Rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

VP2641 Ahn, J. et al. (2013) Mol Cancer Res. 11(8):952. WB: mouse fibroblasts, human H1299 cells
VP2641 Afshari, F.T. et al. (2010) J Neurosci. 30(12):4246. ICC: rat schwann cells

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
Vav (a.a. 165-174) [conserved site] Rabbit pAb50 μlWB, E, IP, ICCHu, Rt, Ms100
Vav2 (Tyr-142)[conserved site], phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck100
Vav2 (Tyr-172)[conserved site], phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms100
Vav2 (a.a. 309-322) Rabbit pAb50 μlWB, EHu, Rt, Ms, Ck100
Vav3 (a.a. 293-305) Rabbit pAb50 μlWB, EHu, Rt, Ms98
KIT SUMMARY

The Vav phospho-regulation antibody sampler kit can be used to examine the level of phosphorylation of Vav2 (Tyr-142) and Vav2 (Tyr-172), as well as the conserved sites in Vav1 and Vav2. In addition, antibodies are provided to monitor the total expression level of Vav1, Vav2, and Vav3.