Catalog # VK6020

Vav Phospho-Regulation Antibody Kit

Antibody Sampler Kit

Application / Dilution

Species Reactivity

Hu, Rt, Ms, Ck




The Vav phospho-regulation antibody sampler kit can be used to examine the level of phosphorylation of Vav2 (Tyr-142) and Vav2 (Tyr-172), as well as the conserved sites in Vav1 and Vav2. In addition, antibodies are provided to monitor the total expression level of Vav1, Vav2, and Vav3.

Western blot of human A431 cells treated with EGF (lanes 1, 3, & 5) then the blot was exposed to alkaline phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Vav (a.a. 165-174) (lanes 1 & 2), anti-Vav2 (Tyr-142) (lanes 3 & 4), or anti-Vav2 (Tyr-172) (lanes 5 & 6).

Western blot of human Jurkat (lanes 1 & 4), HUVEC (lanes 2 & 5), and A431 (lanes 3 & 6) cells. The blots were probed with anti-Vav2 (a.a. 309-322) at a dilution of 1:500 (lanes 1-3) and anti-Vav3 (a.a. 293-305) at 1:500 (lanes 4-6).

Rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

VP2641 Ahn, J. et al. (2013) Mol Cancer Res. 11(8):952. WB: mouse fibroblasts, human H1299 cells
VP2641 Afshari, F.T. et al. (2010) J Neurosci. 30(12):4246. ICC: rat schwann cells

This kit contains:

Vav (a.a. 165-174) [conserved site] Rabbit pAb50 μlWB, E, IP, ICCHu, Rt, Ms100
Vav2 (Tyr-142)[conserved site], phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck100
Vav2 (Tyr-172)[conserved site], phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms100
Vav2 (a.a. 309-322) Rabbit pAb50 μlWB, EHu, Rt, Ms, Ck100
Vav3 (a.a. 293-305) Rabbit pAb50 μlWB, EHu, Rt, Ms98