The transient receptor potential melastatin (TRPM) subfamily of cation-permeable TRP channels is ubiquitously expressed in mammalian tissues. This family includes TRPM1-8. In addition to acting as a calcium-permeant channel, TRPM6 and TRPM7 possess an inherent serine/threonine kinase activity. TRPM7 specifically is involved with cellular magnesium homeostasis and neurotransmitter release. Due to the magnesium inhibition, TRPM7’s ion channel activity is very low. TRPM7 has been implicated in cell proliferation and migration during cancer progression, and its expression levels correlate with prognosis in breast cancer. TRPM7 kinase activation leads to massive autophosphorylation of the C-terminal region, including phosphorylation of Ser-1493, Ser-1513, and Ser-1569. Both Ser-1513 and Ser-1569 phosphorylation is required for kinase activity, and phosphorylation of Ser-1513 may inhibit Caspase-mediated cleavage of the C-terminal tail. Thus, TRPM7 is a multifunctional transmembrane protein with roles in cell signaling, proliferation, migration, and death.
Clark, K. et al. (2008) PLoS ONE 3(3): e1876
Desai, BN. et al. (2012) Dev. Cell. 22(6): 1149
Park, HS, et al. (2014) Korean J Phys Pharm. 18(1):15-23
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
This kit contains:
|CATALOG#||DESCRIPTION||SIZE||APPLICATIONS||SPECIES REACTIVITY||MW (kDa)|
|TRPM7 (Extracellular region) Mouse mAb||50 μl||WB, E||Hu, Rt, Ms||220|
|TRPM7 (Extracellular region) Rabbit pAb||50 μl||WB, E||Hu, Rt, Ms||220|
|TRPM7 (a.a. 1484-1497) Rabbit pAb||50 μl||WB, E||Hu||220|
|TRPM7 (Ser-1493), phospho-specific Rabbit pAb||50 μl||WB, E||Hu||220|
|TRPM7 (Ser-1513), phospho-specific Rabbit pAb||50 μl||WB, E||Hu, Rt, Ms||220|
|TRPM7 (Ser-1569), phospho-specific Rabbit pAb||50 μl||WB, E||Hu, Rt, Ms||220|
The TRPM7 phospho-regulation antibody sampler kit includes phospho-specific antibodies for detection of the phosphorylation of Ser-1493, Ser-1513, and Ser-1569 relative to total TRPM7 expression levels. The kit includes rabbit polyclonal and mouse monoclonal antibodies to TRPM7 for examining total expression level of TRPM7.