Catalog # TK6990

TRPM7 Phospho-Regulation Antibody Kit

Antibody Sampler Kit

DATASHEET  




$455

The transient receptor potential melastatin (TRPM) subfamily of cation-permeable TRP channels is ubiquitously expressed in mammalian tissues. This family includes TRPM1-8. In addition to acting as a calcium-permeant channel, TRPM6 and TRPM7 possess an inherent serine/threonine kinase activity. TRPM7 specifically is involved with cellular magnesium homeostasis and neurotransmitter release. Due to the magnesium inhibition, TRPM7’s ion channel activity is very low. TRPM7 has been implicated in cell proliferation and migration during cancer progression, and its expression levels correlate with prognosis in breast cancer. TRPM7 kinase activation leads to massive autophosphorylation of the C-terminal region, including phosphorylation of Ser-1493, Ser-1513, and Ser-1569. Both Ser-1513 and Ser-1569 phosphorylation is required for kinase activity, and phosphorylation of Ser-1513 may inhibit Caspase-mediated cleavage of the C-terminal tail. Thus, TRPM7 is a multifunctional transmembrane protein with roles in cell signaling, proliferation, migration, and death.

References
Masayuki, M. et al. (2005) J of Bio Chem 280(21): 20793
Clark, K. et al. (2008) PLoS ONE 3(3): e1876
Desai, BN. et al. (2012) Dev. Cell. 22(6): 1149
Park, HS, et al. (2014) Korean J Phys Pharm. 18(1):15-23

Western blot image of human autophosphorylated TRPM7 C-terminal kinase domain (lanes 1-6). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 phosphosites (lanes 2, 4, & 6). The blot was probed with rabbit polyclonals anti-TRPM7 (Ser-1513) phospho-specific (lanes 1 & 2), anti-TRPM7 (a.a.1484-1497) TP5691 antibody (lanes 3 & 4), or anti-TRPM7 (Ser-1493) phospho-specific (lanes 5 & 6).

Western blot image of TRPM7 expression in human MCF-7 cell lysate. The blot was probed with mouse monoclonal anti-TRPM7 (Extracellular region) M573 at a dilution of 1:250.



Rabbit polyclonal and mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
TRPM7 (Extracellular region) Mouse mAb50 μlWB, EHu, Rt, Ms220
TRPM7 (Extracellular region) Rabbit pAb50 μlWB, EHu, Rt, Ms220
TRPM7 (a.a. 1484-1497) Rabbit pAb50 μlWB, EHu220
TRPM7 (Ser-1493), phospho-specific Rabbit pAb50 μlWB, EHu220
TRPM7 (Ser-1513), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms220
TRPM7 (Ser-1569), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms220
KIT SUMMARY

The TRPM7 phospho-regulation antibody sampler kit includes phospho-specific antibodies for detection of the phosphorylation of Ser-1493, Ser-1513, and Ser-1569 relative to total TRPM7 expression levels. The kit includes rabbit polyclonal and mouse monoclonal antibodies to TRPM7 for examining total expression level of TRPM7.