Catalog # TK6470
Talin Phospho-Regulation Antibody Kit
Antibody Sampler Kit
| ELISA, WB, AB, IP, ICC, IHC |
Species Reactivity
| Hu, Rt, Ms, Rb, Ck, F |
 |
KIT SUMMARY The talin phospho-regulation antibody sampler kit can be used to detect the level of phosphorylation of talin (Ser-425) relative to the total expression level of talin. The kit also includes phosho-talin (Ser-425) peptide for antibody blocking experiments and secondary reagents for antibody detection. |
Western blot of rat PC12 cells stimulated with Calyculin A (100 nM) for 30 min (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal anti-Talin (8D4) (lanes 1 & 2) or rabbit polyclonal anti-Talin (Ser-425) (lanes 3 & 4) antibodies.
Immunocytochemical labeling of Talin relative to F-actin in chick fibroblasts. The cells were labeled with mouse monoclonal Talin (Rod domain) antibody (TM4081), then the antibody was detected using appropriate secondary antibody (Red). This labeling is compared to F-actin staining (Green). (Image provided by Dr. Gianluca Gallo at Drexel University).
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
TP4171 Jin, J.K. et al. (2015) Oncogene. 34(14): 1811. WB: metastatic PCa cells, PC3-MM2, shRNATX4175 Jin, J.K. et al. (2015) Oncogene. 34(14): 1811. WB: metastatic PCa cells, PC3-MM2, shRNA
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| Talin (Rod domain) Mouse mAb | 50 μl | WB, E, IP, ICC | Hu, Rt, Ms, Ck, F | 240 | |
| Talin (Ser-425), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, Ck, F | 240 | |
| phospho-Talin (Ser-425) Peptide | 50 μg | AB, E | |||
| Anti-Mouse Ig:HRP Donkey pAb | 100 μl | WB, E | Ms | ||
| Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb | 100 μl | WB, E, ICC, IHC | Rb |
