Catalog # SK7750

Sema-3A & NRP1/Plexin A1 Receptor Staining Kit

Immunocytochemistry Kit

DATASHEET  




$405

One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin-1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A-containing region. Thus, Sema3A and its co-receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.

References
Tamagnone, L. et al. (1999) Cell 99:71.
Takahashi, T. & Strittmatter, S.M. (2001) Neuron 29:429.
Pasterkamp, R.J. & Verhaagen, J. (2001) Brain Res Rev 35:36.
Nakamura, F. & Goshima, Y. (2002) Adv Exp Med Biol. 515:55.

Immunocytochemical labeling of Sema-3A and its receptors in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-Sema-3A (C-terminal) (SP1221), anti-NRP1 (NP221), and anti-Plexin A1 (PP1301). The antibodies were detected using appropriate secondary antibody conjugated to either DyLight® 488 or DyLight® 594.



Rabbit polyclonal antibodies and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

NP2111 Boro, A. et al. (2015) Am J Transl Res. 7(3):640-653 WB: human osteosarcoma cell lines
PP1301 Cheadle, L. et al. (2014) J of Neuroscience 34(23): 7999. WB: rat brain
NP2111 Ohkusu-Tsukada, K. et al. (2010) Int J Cancer. Epub 15 MAR. ELISA: sNRP1 mouse serum
NP2111 Drenberg, C.D. et al. (2009) Obstet Gynecol Intl. 2009:1-8. IHC: human ovarian tissues
SP1221 Pan, H. et al. (2009) Breast Can Res Treat. 118(1):197. WB: MDA-MB-231, shRNA knockdown
NP2111 Saban, M.R. et al. (2008) AJP Renal Phys. 295(1):F60. ICC: human urothelium
SP1221 Tang, X-Q. et al. (2007) J. Neurosci. 27(22):6068. WB: rat E14 spinal cord

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYICC DILUTION
Neuropilin-1 (a1 CUB domain) Rabbit pAb50 μlWB, E, ICC, IHCHu, Rt, Ms1:200
Plexin A1 (Sema domain) Rabbit pAb50 μlWB, E, IP, ICC, IHCHu, Rt, Ms 1:100
Semaphorin-3A (Central region) Rabbit pAb50 μlWB, E, ICC, IHCHu, Rt, Ms1:100
Anti-Rabbit Ig:DyLight® 488 Goat pAb100 μlICC, IHCRb1:200
Anti-Rabbit Ig:DyLight® 594 Goat pAb100 μlICC, IHCRb1:200
KIT SUMMARY

The Sema-3A & NRP1/Plexin A1 receptor kit can be used for immuncytochemical labeling of Sema-3A relative to its receptors, NRP1 and Plexin A1. The kit also includes secondary reagents conjugated to DyLight® 488 or DyLight® 594 for detection of primary antibodies using green (493Ex/518Em) or red (593Ex/618Em) filter sets.