One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin-1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A-containing region. Thus, Sema3A and its co-receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.
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Pasterkamp, R.J. & Verhaagen, J. (2001) Brain Res Rev 35:36.
Nakamura, F. & Goshima, Y. (2002) Adv Exp Med Biol. 515:55.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:NP2111 Boro, A. et al. (2015) Am J Transl Res. 7(3):640-653 WB: human osteosarcoma cell lines
PP1301 Cheadle, L. et al. (2014) J of Neuroscience 34(23): 7999. WB: rat brain
NP2111 Ohkusu-Tsukada, K. et al. (2010) Int J Cancer. Epub 15 MAR. ELISA: sNRP1 mouse serum
NP2111 Drenberg, C.D. et al. (2009) Obstet Gynecol Intl. 2009:1-8. IHC: human ovarian tissues
SP1221 Pan, H. et al. (2009) Breast Can Res Treat. 118(1):197. WB: MDA-MB-231, shRNA knockdown
NP2111 Saban, M.R. et al. (2008) AJP Renal Phys. 295(1):F60. ICC: human urothelium
SP1221 Tang, X-Q. et al. (2007) J. Neurosci. 27(22):6068. WB: rat E14 spinal cord
This kit contains:
|CATALOG#||DESCRIPTION||SIZE||APPLICATIONS||SPECIES REACTIVITY||ICC DILUTION|
|Neuropilin-1 (a1 CUB domain) Rabbit pAb||50 μl||WB, E, ICC, IHC||Hu, Rt, Ms||1:200|
|Plexin A1 (Sema domain) Rabbit pAb||50 μl||WB, E, IP, ICC, IHC||Hu, Rt, Ms||1:100|
|Semaphorin-3A (Central region) Rabbit pAb||50 μl||WB, E, ICC, IHC||Hu, Rt, Ms||1:100|
|Anti-Rabbit Ig:DyLight® 488 Goat pAb||100 μl||ICC, IHC||Rb||1:200|
|Anti-Rabbit Ig:DyLight® 594 Goat pAb||100 μl||ICC, IHC||Rb||1:200|
The Sema-3A & NRP1/Plexin A1 receptor kit can be used for immuncytochemical labeling of Sema-3A relative to its receptors, NRP1 and Plexin A1. The kit also includes secondary reagents conjugated to DyLight® 488 or DyLight® 594 for detection of primary antibodies using green (493Ex/518Em) or red (593Ex/618Em) filter sets.