Catalog # SK7750
Sema-3A & NRP1/Plexin A1 Receptor Staining Kit
Immunocytochemistry Kit
| ELISA, WB, IP, ICC, IHC |
Species Reactivity
| Hu, Rt, Ms, Rb |
 |
KIT SUMMARY The Sema-3A & NRP1/Plexin A1 receptor kit can be used for immuncytochemical labeling of Sema-3A relative to its receptors, NRP1 and Plexin A1. The kit also includes secondary reagents conjugated to DyLight® 488 or DyLight® 594 for detection of primary antibodies using green (493Ex/518Em) or red (593Ex/618Em) filter sets. |
Immunocytochemical labeling of Sema-3A and its receptors in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-Sema-3A (C-terminal) (SP1221), anti-NRP1 (NP2111), and anti-Plexin A1 (PP1301). The antibodies were detected using appropriate secondary antibody conjugated to either DyLight® 488 or DyLight® 594.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
NP2111 Boro, A. et al. (2015) Am J Transl Res. 7(3):640-653 WB: human osteosarcoma cell linesPP1301 Cheadle, L. et al. (2014) J of Neuroscience 34(23): 7999. WB: rat brain
NP2111 Ohkusu-Tsukada, K. et al. (2010) Int J Cancer. Epub 15 MAR. ELISA: sNRP1 mouse serum
NP2111 Drenberg, C.D. et al. (2009) Obstet Gynecol Intl. 2009:1-8. IHC: human ovarian tissues
SP1221 Pan, H. et al. (2009) Breast Can Res Treat. 118(1):197. WB: MDA-MB-231, shRNA knockdown
NP2111 Saban, M.R. et al. (2008) AJP Renal Phys. 295(1):F60. ICC: human urothelium
SP1221 Tang, X-Q. et al. (2007) J. Neurosci. 27(22):6068. WB: rat E14 spinal cord
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | ICC DILUTION |
| Neuropilin-1 (a1 CUB domain) Rabbit pAb | 50 μl | WB, E, ICC, IHC | Hu, Rt, Ms | 1:200 | |
| Plexin A1 (Sema domain) Rabbit pAb | 50 μl | WB, E, IP, ICC, IHC | Hu, Rt, Ms | 1:100 | |
| Semaphorin-3A (Central region) Rabbit pAb | 50 μl | WB, E, ICC, IHC | Hu, Rt, Ms | 1:100 | |
| Anti-Rabbit Ig:DyLight® 488 Goat pAb | 100 μl | ICC, IHC | Rb | 1:200 | |
| Anti-Rabbit Ig:DyLight® 594 Goat pAb | 100 μl | ICC, IHC | Rb | 1:200 |
