Catalog # SK6710


Sox2 Phospho-/Methyl-Specific

Antibody Sampler Kit

$405

Embryonic stem cells can maintain a pluripotent state that is controlled by a set of transcription factors that include Oct-4, Sox2, and Nanog. Chromatin immunoprecipitation experiments show that Sox2 and Oct-4 bind to thousands of gene regulatory sites, many of which regulate cell pluripotency and early embryonic development. siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency, while overexpression of Oct-4 and Sox2, along with additional transcription factors Klf4 and c-Myc, can reprogram somatic cells to a pluripotent state. Sox2 also regulates adult multipotent progenitors in various epithelial tissues, and may be important for survival and regeneration of these tissues. The activity of Sox2 may be regulated by phosphorylation and methylation. Akt1 phosphorylates Thr-118 and enhances Sox2 transcriptional activity, while Set7 can monomethylate Lys-119 leading to inhibition of Sox2 transcriptional activity, as well as Sox2 ubiquination and degradation. In addition, Sox2 Thr-128 is constituitively phoshorylated in the F9 mouse stem cell line.

References
Boyer, L.A. et al. (2005) Cell. 122:947.
Loh, Y.H. et al. (2006) Nat Genet. 38:431.
Jeong, C.H. et al. (2010) Stem Cells. 28(12):2141.
Fang, L.. et al. (2014) Mol Cell. 55(4):537.
Primary antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

SP5521 Mir S. et al. (2017) J Biol Chem. 292(6):2054. ICC: mouse hippocampal stem cells
SP5521 Mir S. et al. (2017) Sci Rep. 7(1):3283. WB & ICC: mouse hippocampal stem cells
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot

Western blot image of mouse F9 stem cells treated with with calyculin A (100 nM, 30 min.) (lanes 1-4) then Sox2 was dephosphorylated with lambda phosphatase (lanes 2 & 4). The blot was probed with mouse monoclonal Sox2 (lanes 1 & 2) and rabbit polyclonal anti-Sox2 (Thr-118) phospho-specific antibody (lanes 3 & 4).

Western blot image of human Sox2 recombinant protein untreated (lanes 1 & 3) or treated with Set7 methyltransferase to methylate Lys-119 (lanes 2 & 4). The blot was probed with mouse monoclonal Sox2 (lanes 1 & 2) and rabbit polyclonal anti-Sox2 (Lys-119) methyl-specific antibody (lanes 3 & 4).



This kit contains:

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CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
Sox2 Mouse mAb50 μlWB, E, ICCHu, Rt, Ms, Ck, F35
Sox2 (Thr-118), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck, F35
Sox2 (Lys-119), methyl-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck, F35
Sox2 (Thr-128), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck, F35
Anti-Mouse Ig:HRP Donkey pAb100 μlWB, E, ICC, IHCMs
Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb100 μlWB, E, ICC, IHCRb
KIT SUMMARY

The Sox2 Phospho-/Methyl-Specific Antibody Sampler Kit can be used to detect phosphorylation of Thr-118 and Thr-128, as well as methylation of Lys-119 relative to total Sox2 expression levels. The kit includes rabbit polyclonal antibodies to phospho-Thr-118, phospho-Thr-128, and methyl-Lys-119, as well as a Sox2 mouse monoclonal antibody. The kit also includes anti-Rabbit Ig Light-Chain Specific:HRP and anti-Mouse Ig:HRP secondary reagents for detection of antibodies in Western blot, ELISA, or immunocytochemistry.