Catalog # SK6710
Sox2 Phospho-/Methyl-Specific Antibody Kit
Antibody Sampler Kit
| ELISA, WB, ICC, IHC |
Species Reactivity
| Hu, Rt, Ms, Rb, Ck, F |
 |
KIT SUMMARY The Sox2 Phospho-/Methyl-Specific Antibody Sampler Kit can be used to detect phosphorylation of Thr-118 and Thr-128, as well as methylation of Lys-119 relative to total Sox2 expression levels. The kit includes rabbit polyclonal antibodies to phospho-Thr-118, phospho-Thr-128, and methyl-Lys-119, as well as a Sox2 mouse monoclonal antibody. The kit also includes anti-Rabbit Ig Light-Chain Specific:HRP and anti-Mouse Ig:HRP secondary reagents for detection of antibodies in Western blot, ELISA, or immunocytochemistry. |
Western blot image of mouse F9 stem cells treated with with calyculin A (100 nM, 30 min.) (lanes 1-4) then Sox2 was dephosphorylated with lambda phosphatase (lanes 2 & 4). The blot was probed with mouse monoclonal Sox2 (lanes 1 & 2) and rabbit polyclonal anti-Sox2 (Thr-118) phospho-specific antibody (lanes 3 & 4).
Western blot image of human Sox2 recombinant protein untreated (lanes 1 & 3) or treated with Set7 methyltransferase to methylate Lys-119 (lanes 2 & 4). The blot was probed with mouse monoclonal Sox2 (lanes 1 & 2) and rabbit polyclonal anti-Sox2 (Lys-119) methyl-specific antibody (lanes 3 & 4).
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
SP5521 Mir S. et al. (2017) J Biol Chem. 292(6):2054. ICC: mouse hippocampal stem cellsSP5521 Mir S. et al. (2017) Sci Rep. 7(1):3283. WB & ICC: mouse hippocampal stem cells
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| Sox2 Mouse mAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, Ck, F | 35 | |
| Sox2 (Thr-118), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, Ck, F | 35 | |
| Sox2 (Lys-119), methyl-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, Ck, F | 35 | Sox2 (Thr-128), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, Ck, F | 35 |
| Anti-Mouse Ig:HRP Donkey pAb | 100 μl | WB, E, ICC, IHC | Ms | ||
| Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb | 100 μl | WB, E, ICC, IHC | Rb |
