Catalog # SK6610

Semaphorins 3A, 3E, 3F, and 4D Antibody Kit

Antibody Sampler Kit

Application / Dilution

Species Reactivity

Hu, Rt, Ms, Ck




The Semaphorin antibody kit can be used to compare the expression of Sema-3A, Sema-3E, Sema-3F, and Sema-4D in cells and tissues. The kit includes three Sema-3A antibodies that target the N-terminal, Central, and C-terminal regions of Sema-3A.

Western blots of neonatal rat brain (lanes 1, 3, & 5) and human recombinant Sema3A/Fc chimera (95/125 kDa) (lanes 2, 4, & 6). Blots were probed with anti-Sema3A (SP1401) (lanes 1 & 2), anti-Sema3A (SP1221) (lanes 3 & 4) and anti-Sema3A (SP1241) (lane 5 & 6).

Western blot image of mouse brain lysate. The blot was probed with mouse monoclonal Semaphorin-4D antibody (SM1881) at 1:250 (lane 1), 1:500 (lane 2), and 1:1000 (lane 3).

Rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

SM1881 Damola, A. et al. (2013) Prostate 73(12):1326. WB: prostate cancer cells
SP1221 Pan, H. et al. (2009) Breast Can Res Treat. 118(1):197. WB: MDA-MB-231, shRNA knockdown
SP1221 Tang, X-Q. et al. (2007) J. Neurosci. 27(22):6068. WB: rat E14 spinal cord

This kit contains:

Semaphorin-3A (Central region) Rabbit pAb50 μlWB, E, ICC, IHCHu, Rt, Ms95
Semaphorin-3A (C-terminal) Rabbit pAb50 μlWB, EHu95
Semaphorin-3A (N-terminal) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms95
Semaphorin-3E (N-terminal region) Rabbit pAb50 μlWB, EHu, Rt, Ms, Ck87
Semaphorin-3F (a.a. 746-762) Rabbit pAb50 μlWB, EHu, Rt, Ms95
Semaphorin-4D (C-terminal) Mouse mAb50 μlWB, E, ICCHu, Rt, Ms150