One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that have been implicated in neuron development, vascular disease, and tumor progression. There are eight classes of semaphorin genes, all of which are characterized by a conserved 500 amino acid, cystine-rich Sema domain. Semaphorin 3A (Sema-3A) binds its co-receptors, Neuropilin-1 and Plexin A1, and this interaction has been implicated in suppressing axon regeneration after injury. Sema-3E binds Plexin D1 receptor, and has putative roles in axon guidance, angiogenesis, and cancers. The Sema-3E protein is expressed as full length (87 kDa) and furin-dependent processed forms (61 and 25 kDa). Sema-3F binds with high affinity to Neuropilin-2, and low affinity to Neuropilin-1. Sema-3F has been implicated in neuronal development, angiogenesis, and tumor progression. Sema-4D binds Plexin B1, and this pathway may be involved in cell survival and angiogenesis.
Kolk, S.M. et al. (2009) J Neurosci. 29(40):12542.
Tran, T.S. et al. (2009) Nature. 462(7276):1065.
Casazza, A. et al. (2010) J Clin Invest. 120(8):2684.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:SM1881 Damola, A. et al. (2013) Prostate 73(12):1326. WB: prostate cancer cells
SP1221 Pan, H. et al. (2009) Breast Can Res Treat. 118(1):197. WB: MDA-MB-231, shRNA knockdown
SP1221 Tang, X-Q. et al. (2007) J. Neurosci. 27(22):6068. WB: rat E14 spinal cord
This kit contains:
|CATALOG#||DESCRIPTION||SIZE||APPLICATIONS||SPECIES REACTIVITY||MW (kDa)|
|Semaphorin-3A (Central region) Rabbit pAb||50 μl||WB, E, ICC, IHC||Hu, Rt, Ms||95|
|Semaphorin-3A (C-terminal) Rabbit pAb||50 μl||WB, E||Hu||95|
|Semaphorin-3A (N-terminal) Rabbit pAb||50 μl||WB, E, ICC||Hu, Rt, Ms||95|
|Semaphorin-3E (N-terminal region) Rabbit pAb||50 μl||WB, E||Hu, Rt, Ms, Ck||87|
|Semaphorin-3F (a.a. 746-762) Rabbit pAb||50 μl||WB, E||Hu, Rt, Ms||95|
|Semaphorin-4D (C-terminal) Mouse mAb||50 μl||WB, E, ICC||Hu, Rt, Ms||150|
The Semaphorin antibody kit can be used to compare the expression of Sema-3A, Sema-3E, Sema-3F, and Sema-4D in cells and tissues. The kit includes three Sema-3A antibodies that target the N-terminal, Central, and C-terminal regions of Sema-3A.