Catalog # SK6610

Semaphorins 3A, 3E, 3F, and 4D Antibody Kit

Antibody Sampler Kit



One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that have been implicated in neuron development, vascular disease, and tumor progression. There are eight classes of semaphorin genes, all of which are characterized by a conserved 500 amino acid, cystine-rich Sema domain. Semaphorin 3A (Sema-3A) binds its co-receptors, Neuropilin-1 and Plexin A1, and this interaction has been implicated in suppressing axon regeneration after injury. Sema-3E binds Plexin D1 receptor, and has putative roles in axon guidance, angiogenesis, and cancers. The Sema-3E protein is expressed as full length (87 kDa) and furin-dependent processed forms (61 and 25 kDa). Sema-3F binds with high affinity to Neuropilin-2, and low affinity to Neuropilin-1. Sema-3F has been implicated in neuronal development, angiogenesis, and tumor progression. Sema-4D binds Plexin B1, and this pathway may be involved in cell survival and angiogenesis.

Basile, J.R. et al. (2006) PNAS 103(24):9017.
Kolk, S.M. et al. (2009) J Neurosci. 29(40):12542.
Tran, T.S. et al. (2009) Nature. 462(7276):1065.
Casazza, A. et al. (2010) J Clin Invest. 120(8):2684.

Western blots of neonatal rat brain (lanes 1, 3, & 5) and human recombinant Sema3A/Fc chimera (95/125 kDa) (lanes 2, 4, & 6). Blots were probed with anti-Sema3A (SP1401) (lanes 1 & 2), anti-Sema3A (SP1221) (lanes 3 & 4) and anti-Sema3A (SP1241) (lane 5 & 6).

Western blot image of mouse brain lysate. The blot was probed with mouse monoclonal Semaphorin-4D antibody (SM1881) at 1:250 (lane 1), 1:500 (lane 2), and 1:1000 (lane 3).

Rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

SM1881 Damola, A. et al. (2013) Prostate 73(12):1326. WB: prostate cancer cells
SP1221 Pan, H. et al. (2009) Breast Can Res Treat. 118(1):197. WB: MDA-MB-231, shRNA knockdown
SP1221 Tang, X-Q. et al. (2007) J. Neurosci. 27(22):6068. WB: rat E14 spinal cord

This kit contains:

Semaphorin-3A (Central region) Rabbit pAb50 μlWB, E, ICC, IHCHu, Rt, Ms95
Semaphorin-3A (C-terminal) Rabbit pAb50 μlWB, EHu95
Semaphorin-3A (N-terminal) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms95
Semaphorin-3E (N-terminal region) Rabbit pAb50 μlWB, EHu, Rt, Ms, Ck87
Semaphorin-3F (a.a. 746-762) Rabbit pAb50 μlWB, EHu, Rt, Ms95
Semaphorin-4D (C-terminal) Mouse mAb50 μlWB, E, ICCHu, Rt, Ms150

The Semaphorin antibody kit can be used to compare the expression of Sema-3A, Sema-3E, Sema-3F, and Sema-4D in cells and tissues. The kit includes three Sema-3A antibodies that target the N-terminal, Central, and C-terminal regions of Sema-3A.