Catalog # SK6590
Sphingosine Kinase 2 Phospho-Regulation Antibody Kit
Antibody Sampler Kit
| ELISA, WB, IP, ICC, IHC, AB |
Species Reactivity
| Hu, Rt, Ms, Rb |
 |
KIT SUMMARY The Sphingosine Kinase 2 (SK2) phospho-regulation antibody kit can be used to detect phosphorylation of Thr-578 relative to total SK2 expression. The kit includes rabbit polyclonal antibodies, blocking peptides, and secondary reagents for detection in western blot. |
Western blot of human recombinant SK2 (lanes 1-4). The blots were untreated (lane 1 & 3) or treated with lambda phosphatase (lane 2 & 4), then probed with anti-SK2 (N-terminal region) (lanes 1 & 2) or anti-SK2 (Thr-578) (lanes 3 & 4).
Western blot of HeLa treated with calyculin A (lanes 1-4). The blots were untreated (lane 1 & 3) or treated with lambda phosphatase (lane 2 & 4), then probed with anti-SK2 (N-terminal region) (lanes 1 & 2) or anti-SK2 (Thr-578) (lanes 3 & 4).
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
SP4621 Wang, X. et al. (2016) PLoS Pathog. 12(10):e1005926. WB: Brain endothelial cellsSP4631 Wang, X. et al. (2016) PLoS Pathog. 12(10):e1005926. WB: Brain endothelial cells
SP4621 Neubauer, H & Pitson, SM (2016) F1000Research. 5:2825. IP-WB, ICC: HEK293, HeLa, MEF
SP4621 Brunno, G et al. (2015) BBA MCB Lipids. 1851.2:194. WB: murine myoblast
SP4621 St Patrick, R. et al. (2015) Emerg micro & infect. 4(10):e61 ICC: hSKMC, MCF10A
SP4621 Wallington-Beddoe, CT et al. (2014) Cancer Res 74(10):2803. WB: ALL cell lines, K562 siRNA KD
SP4631 Wallington-Beddoe, CT et al. (2014) Cancer Res 74(10):2803. WB: ALL cell lines
SP4631 Dai, L. et al. (2013) Molec Endocrinology 28(2):197. WB: MCF7 + Insulin
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| Sphingosine Kinase 2 (N-terminal region) Rabbit pAb | 50 μl | WB, E, IP, ICC | Hu, Rt, Ms | 70 | |
| Sphingosine Kinase 2 (Thr-578), phospho-specific Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 70 | |
| Sphingosine Kinase 2 (N-terminal region) Peptide | 50 μg | AB, E | |||
| phospho-Sphingosine Kinase 2 (Thr-578) Peptide | 50 μg | AB, E | |||
| Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb | 100 μl | WB, E, ICC, IHC | Rb |
