Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. The extracellular region of plexins contains a semaphorin domain, multiple glycine-rich motifs, and MET-related sequences. The cytoplasmic region contains a Sex/Plexin domain and putative tyrosine phosphorylation sites that mediate signal transduction after activation. Plexin D1 is a type I transmembrane protein that may be expressed as multiple isoforms in many cell types, including neurons and endothelial cells. Semaphorin 3E (Sema-3E) and semaphorin 4A can bind Plexin D1, and ligand binding leads to phosphorylation of Tyr-1642 and activation of Plexin D1. Sema-3E and its receptor are important for angiogenesis that occurs during blood vessel development and repair. In cancers, Sema-3E and Plexin D1 are expressed in tumor tissues and cancer cells, and the interaction of these molecules may promote cancer cell migration and metastic spreading. Thus, Plexin D1, and its ligand Sema-3E, may be important regulators of angiogenesis and metastasis.
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The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
This kit contains:
|CATALOG#||DESCRIPTION||SIZE||APPLICATIONS||SPECIES REACTIVITY||MW (kDa)|
|Plexin D1 (Cytoplasmic domain) Rabbit pAb||50 μl||WB, E||Hu, Rt, Ms||250|
|Plexin D1 (Sema domain) Rabbit pAb||50 μl||WB, E||Hu, Rt, Ms||250|
|Plexin D1 (a.a. 1635-1647) Rabbit pAb||50 μl||WB, E||Hu, Rt, Ms||250|
|Semaphorin-3E (N-terminal region) Rabbit pAb||50 μl||WB, E||Hu, Rt, Ms,Ck||87|
|Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb||100 μl||WB, E, ICC, IHC||Rb|
The Sema-3E and Plexin D1 Receptor kit can be used to monitor the expression of Sema-3E and its receptor Plexin D1. The kit has an N-terminal region Sema-3E antibody, and Plexin D1 antibodies for detecting two regions in the cytoplasmic domain and a region in the extracellular Sema domain. The kit also includes secondary reagent for rabbit polyclonal antibody detection.