Catalog # SK6570

Sema-3E and Plexin D1 Receptor Antibody Kit

Antibody Sampler Kit

Size Kit



Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. The extracellular region of plexins contains a semaphorin domain, multiple glycine-rich motifs, and MET-related sequences. The cytoplasmic region contains a Sex/Plexin domain and putative tyrosine phosphorylation sites that mediate signal transduction after activation. Plexin D1 is a type I transmembrane protein that may be expressed as multiple isoforms in many cell types, including neurons and endothelial cells. Semaphorin 3E (Sema-3E) and semaphorin 4A can bind Plexin D1, and ligand binding leads to phosphorylation of Tyr-1642 and activation of Plexin D1. Sema-3E and its receptor are important for angiogenesis that occurs during blood vessel development and repair. In cancers, Sema-3E and Plexin D1 are expressed in tumor tissues and cancer cells, and the interaction of these molecules may promote cancer cell migration and metastic spreading. Thus, Plexin D1, and its ligand Sema-3E, may be important regulators of angiogenesis and metastasis.

van der Zwaag, B. et al. (2002) Dev Dyn. 2002 Nov;225(3):336-43.
Deutsch, U. (2004) Dev Cell. 7(1):1.
Gu, C. et al. (2005) Science. 307(5707):265.
Casazza, A. et al. (2010) J Clin Invest. 120(8):2684.

Western blot analysis of Plexin D1 expression in human endothelial cells (HUVEC) (lanes 1-6). The blots were probed with rabbit polyclonals anti-Plexin D1 (Sema domain) (lanes 1 & 2), anti-Plexin D1 (Cytoplasmic domain) (lanes 3 & 4), and anti-Plexin D1 (a.a. 1635-1647) (lanes 5 & 6). Each antibody was used in the presence of their respective blocking peptide (lanes 2, 4 & 6).

Western blot analysis of rat PC12 cells (lanes 1 & 2) and human PC-3 cells (lanes 3 & 4). The blots were probed with anti-Sema-3E (N-terminal region) antibody in absence (lanes 1 & 3) or presence of Sema-3E (N-terminal region) blocking peptide (lanes 2 & 4).

Rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot

This kit contains:

Plexin D1 (Cytoplasmic domain) Rabbit pAb50 μlWB, EHu, Rt, Ms250
Plexin D1 (Sema domain) Rabbit pAb50 μlWB, EHu, Rt, Ms250
Plexin D1 (a.a. 1635-1647) Rabbit pAb50 μlWB, EHu, Rt, Ms250
Semaphorin-3E (N-terminal region) Rabbit pAb 50 μlWB, EHu, Rt, Ms,Ck87
Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb100 μlWB, E, ICC, IHCRb