Catalog # SK6420


Semaphorin-3 and Neuropilin

Antibody Sampler Kit

$395

One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that have been implicated in neuron development, vascular disease, and tumor progression. There are eight classes of semaphorin genes, all of which are characterized by a conserved 500 amino acid, cystine-rich Sema domain. Sema-3A and Sema-3F are class III secreted semaphorins that bind with high affinity to Neuropilin-1 and Neuropilin-2, respectively. Both Sema-3A and Sema-3F, as well as their neuropilin receptors, may have important roles in axon guidance, angiogenesis, and tumor progression.

References

Pasterkamp, R.J. & Verhaagen, J. (2001) Brain Res Rev 35:36.
Nakamura, F. & Goshima, Y. (2002) Adv Exp Med Biol. 515:55.
Kolk, S.M. et al. (2009) J Neurosci. 29(40):12542.
Tran, T.S. et al. (2009) Nature. 462(7276):1065.

Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

NP2111 Boro, A. et al. (2015) Am J Transl Res. 7(3):640-653 WB: human osteosarcoma cell lines
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
NP2111 Ohkusu-Tsukada, K. et al. (2010) Int J Cancer. Epub 15 MAR. ELISA: sNRP1 mouse serum
NP2111 Drenberg, C.D. et al. (2009) Obstet Gynecol Intl. 2009:1-8. IHC: human ovarian tissues
SP1221 Pan, H. et al. (2009) Breast Can Res Treat. 118(1):197. WB: MDA-MB-231, shRNA knockdown
NP2111 Saban, M.R. et al. (2008) AJP Renal Phys. 295(1):F60. ICC: human urothelium
SP1221 Tang, X-Q. et al. (2007) J. Neurosci. 27(22):6068. WB: rat E14 spinal cord

Western blot analysis of Sema-3F expression in adult mouse brain (lane 1), kidney (lane 2), and liver (lane 3). The blot was probed with anti-Sema-3F (SP3921) at 1:1000.

Formalin fixed, citric acid treated parafin sections of adult Rat cerebellum. Sections were probed with anti-Sema3A (SP1221) then anti-Rabbit:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).



This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
Neuropilin-1 (a1 CUB domain) Rabbit pAb50 μlWB, E, ICC, IHCHu, Rt, Ms130
Neuropilin-2 (MAM domain) Mouse mAb50 μlWB, EHu, Rt, Ms130
Semaphorin-3A (Central region) Rabbit pAb50 μlWB, E, ICC, IHCHu, Rt, Ms95
Semaphorin-3F (a.a. 746-762) Rabbit pAb50 μlWB, EHu, Rt, Ms95
Anti-Mouse Ig:HRP Donkey pAb 100 μlWB, EMs
Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb 100 μlWB, E, ICC, IHCRb
KIT SUMMARY

The Semaphorin-3 and Neuropilin antibody sampler kit can be used to examine the expression of semaphorin 3A (Sema-3A) and 3F (Sema-3F), and their receptors, Neuropilin 1 (NRP1) and Neuropilin 2 (NRP2). Included in the kit are secondary reagents for detection of rabbit polyclonal and mouse monoclonal antibodies.