Catalog # SK6410

Slingshot-1L Phospho-Regulationv

Antibody Sampler Kit

DATASHEET  




$395

Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cell motility. Regulation of cofilin activity can occur through serine phosphorylation and dephosphorylation. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. Multiple phosphatases, Slingshot, PP1, PP2A, PP2B, and chronophin can dephosphorylate Ser-3 and activate actin binding. In mammals, the Slingshot family includes SSH1L, SSH2L, and SSH3L. SSH1L and SSH2L mRNAs are widely expressed, while SSH3L has high expression in epithelial tissues. SSH1L can associate with F-actin and may be the major phosphatase regulating cofilin activity. Disruption of SSH1L expression using RNA interference impairs directional cell migration. Phosphorylation of SSH1L at Ser-937 and Ser-978 by PKD leads to association with 14-3-3, sequestration of the phosphatase to the cytoplasm, and reduces cell migration. Thus, Slingshot phosphatases may be critical for regulating cytoskeletal protein activity and cell motility.

References
Nagata-Ohashi, K. et al. (2004) J Cell Biol. 165(4):465.
Soosairajah, J. et al. (2005) EMBO J. 24(3):473.
Peterburs, P. et al. (2009) Cancer Res. 69(14):5634.
Wallington, C.T. et al. (2014) Cancer Res(74): 2803.

Western blot of human recombinant SSH1L untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1L (C-term.) (lanes 1, 2, 5, & 6) or anti-SSH1L (Ser-978) (lanes 3, 4, 7, & 8).

Immunocytochemical labeling of Slingshot-1L in rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-SSH1L (C-term.) and anti-SSH1L (Ser-978) antibodies, then detected using appropriate secondary antibody conjugated to Cy3 (Right panel). Phase image of corresponding PC12 cells (Left panel).



Rabbit polyclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide, and the peptides are supplied in PBS and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

SP1711 Wang, Y. et al. (2015) Cancer Lett. 360(2):171
SP3901 Fengbo, T. et al. (2015) J Exp Clin Cancer Res. 9(34):117 WB: HCT-116, LoVo, CRC tissues
SP3901 St Patrick, R. et al. (2015)Emerg micro & infect. 4(10): e61 IF: hSKMC, MCF10A
SP3901 Xiu, L. et al. (2015) Am J Pathol. 185(2):387 WB: human MSCs and HPCs
SP3901 Moylan, J. et al. (2014) Redox biology (2)910: 920. WB: C2C12 myoblast
SP1711 Wang, Y. et al. (2013) J Neurosci. 33(15):6423. WB: Rat infralimbic cortex
SP3901 DiPaolo, B.C. et al. (2013) Am Jou of Phys 305(2): L141 WB: rat alveolar type II
SP3901 Land, D. et al. (2013) Molec Endocrin 28(2): 197. WB: MCF7
SP3901 Vitolo, M.I. et al. (2013) Oncogene. 32(17):2200. WB: MCF-10A, 293T
SP3901 Wang, Y. et al. (2013) J Neurosci. 33(15):6423. WB: Rat infralimbic cortex
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
SP3901 Xiang, S.Y. et al. (2013) Sci Signal. 6(306):ra108. WB: rat cardiomyocytes, S1P treated
SP1711 DiPaolo, B.C. et al. (2013) Am Jou of Phys 305(2): L141. WB: rat alveolar type II
SP1711 Sparrow, N. et al. (2012) J Neurosci. 32(15):5284. WB & ICC: Rat Schwann cells
SP3901 Sparrow, N. et al. (2012) J Neurosci. 32(15):5284. WB & ICC: Rat Schwann cells
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
SP1711 Eiseler, T. et al. (2009) Nat Cell Biol. 11(5):545. WB, IP, ICC: human HeLa cells
SP1711 Kim, J.S. et al. (2009) Mol Biol Cell. 20(11):2650. WB: human HeLa, siRNA
SP1711 Yuen, E.Y. & Yan, Z. (2009) J Neurosci. 29:550. ICC: rat interneurons, siRNA

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
Slingshot-1L (C-terminal region) Rabbit pAb50 μlWB, E, ICC, IHCHu, Rt, Ms150
Slingshot-1L (Ser-978), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck150
Slingshot-1L (C-terminal region) Peptide50 μgAB, E
phospho-Slingshot-1L (Ser-978) Peptide50 μgAB, E
Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb100 μlWB, E, ICC, IHCRb
KIT SUMMARY

Rabbit polyclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide, and the peptides are supplied in PBS and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.