Catalog # SK6280

SHP1 Phospho-Regulation Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395

SHP1 (PTP1C, SH-PTP1, or HCP) is a protein-tyrosine phosphatase (PTP) involved in cell migration, cell proliferation, and immune cell function. This phosphatase contains two N-terminal SH2 domains and a C-terminal phosphatase domain. SHP1 associates with a variety of cytokine and growth factor receptors and regulates signal transduction through dephosphorylation of these receptors or their downstream effectors. Downstream of receptor activation, SHP1 regulates the transcriptional activity stimulated by JAK/Stat and MAPK pathways. SHP1 activity is regulated by both tyrosine and serine phosphorylation. Phosphorylation of Tyr-536 and Tyr-564 stimulates phosphatase activity and promotes interaction with Grb-2. Serine phosphorylation at Ser-591 is mediated by PKCα and leads to inhibition of phosphatase activity. Thus, phosphorylation at tyrosine relative to serine residues may be regulated by different cell signaling pathways to control SHP1 activity.

References
Zhang, J. et al. (2000) Semin. Immunol. 12:361.
Zhang, Z. et al. (2003) J. Biol. Chem. 278(7):4668.
Jones, M.L. et al. (2004) J. Biol. Chem. 279(39):40475.

Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to alkaline phosphatase (lanes 2 & 4) then probed with anti-SHP1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP1 (Tyr-536) antibody (lanes 3-5). The SHP1 (Tyr-536) antibody was used in the presence of phospho-SHP1 (Tyr-536) peptide (lane 5).

Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to lambda phosphatase (lanes 2 & 4) then probed with anti-SHP1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP1 (Ser-591) antibody (lanes 3-6). The SHP1 (Ser-591) antibody was used in the presence of phospho-SHP1 (Ser-591) peptide (lane 5) or a non-specific phospho-serine peptide (lane 6).



Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

SP1531 Mani, R. et al. (2015) Leukemia 29: 346. WB: mouse CCL
SP1571 Choia, Y.R. et al. (2015) Neurobiol Dis. 83: 90. IF, WB: BV-2, COS-2, Rat primary microglia
SP1571 Kataoka, T. R. et al. (2015) Int Immunol. 27(2): 95. WB: mouse BMMCs
SP1571 Khan, T. H. et al. (2014) J Immunology 193(7): 3644. WB: mouse P388D1
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
SP1531 Zhou, X. et al. (2013) AJP Renal Phys. 305(3):362. WB Li-Cor: HEK293 cells plus NaCl
SP1531 Sanchez-Quiles, V. et al. (2012) J Proteomics. 75(18):5783. WB: mouse splenocytes
SP1571 Aloulou, M. et al. (2012) Blood. 119(13)3084. WB: rat mast cells
SP1571 Bandyopadhyay, G. et al. (2012) Clin Immunol. 145(1):44. FC: human T-cells
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
SP1531 Salas, A. et al. (2011) Blood. 117(22):5941. WB: human K562 cells
SP1571 Tibaldo, E. et al. (2011) Leukemia. 25(11): 1768. WB: human B-CCL cells
SP1571 Tachdjian et al. (2010) J Aller Clin Imm. 125(5):1128. WB: mouse macrophages
SP1571 Verhagen, A.M. et al. (2009) J. Immunol. 181:2020. WB: mouse B cells
SP1531 Simoneau, M. et al. (2008) J Biol Chem. 283(37):25544. WB: S591A transfectants

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
SHP1 (C-terminal region) Mouse mAb50 μlWB, E, IP, ICCHu, Rt, Ms68
SHP1 (Tyr-536), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms68
SHP1 (Ser-591), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms68
Anti-Mouse Ig:HRP Donkey pAb100 μlWB, EMs
Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb100 μlWB, E, ICC, IHCRb
KIT SUMMARY

The SHP1 phospho-regulation antibody sampler kit can be used to detect SHP1 phosphorylation at Tyr-536 and Ser-591. The kit also includes an antibody to examine total SHP1 expression levels and secondary reagents for rabbit polyclonal and mouse monoclonal antibody detection.