Catalog # SK6240

Sema-4D and Plexin B1 Receptor Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395

Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. The extracellular region of plexins contains a semaphorin domain, multiple glycine-rich motifs, and MET-related sequences. The cytoplasmic region contains a Sex/Plexin domain and putative tyrosine phosphorylation sites that mediate signal transduction after activation. In addition to these motifs, the Plexin B1 and B2 family members contain cleavage sites for subtilisin-like proprotein convertases. These cleavage sites may be important for regulating assembly of heterodimeric receptors or release of the extracellular domain. Plexin-B1 is expressed as a 300 kDa precursor protein that can be cleaved into a 200 kDa a-subunit and a 100 kDa b-subunit. This plexin is the receptor for the class IV semaphorin, Sema-4D. Sema-4D activation of Plexin B1 recruits and activates c-Met, which can promote cell survival, migration, and angiogenesis.

References
Tamagnone, L. et al. (1999) Cell 99:71.
Artigiani, S. et al. (2003) J Biol. Chem. 278(12):10094.
Basile, J.R. et al. (2006) PNAS 103(24):9017.

Western blot image of mouse brain lysate. The blot was probed with mouse monoclonal Semaphorin-4D antibody (SM1881) at 1:250 (lane 1), 1:500 (lane 2), and 1:1000 (lane 3).

Immunocytochemical labeling in rat PC12 cells differentiated with NGF. The cells were probed with rabbit polyclonal Plexin B1 (PP1841) and mouse monoclonal Sema-4D (SM1881) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.



Rabbit polyclonal and mouse monoclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide, and the peptide is supplied in PBS and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

PP1841 Damola, A. et al. (2013) Prostate 73(12):1326. WB, IP: Prostate Cells, shRNA knockdown
PP1841 Soong, J. & Scott, G. (2013) J Cell Sci. 126(Pt 2):688. WB: human melanocytes
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
SM1881 Damola, A. et al. (2013) Prostate 73(12):1326. WB: prostate cancer cells
PP1841 Soong, J. et al (2012) J Invest Dermatol. 132(4):1230. WB: melanocyte, keratinocyte, smooth muscle
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
Plexin B1 (C-terminal region) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms300
Plexin B1 (C-terminal region) Peptide50 μgAB, E
Semaphorin-4D (C-terminal region) Mouse mAb 50 μlWB, E, ICCHu, Rt, Ms150
Anti-Mouse Ig:HRP Donkey pAb100 μlWB, EMs
Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb100 μlWB, E, ICC, IHCRb
KIT SUMMARY

The Sema-4D and Plexin B1 Receptor antibody sampler kit can be used to detect the changes in the expression of Semaphorin 4D and its receptor Plexin B1. The kit also includes a Plexin B1 peptide for antibody blocking experiments and secondary reagents for antibody detection.