Catalog # SK6190

Sema-3A and NRP1/Plexin A1 Receptor Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395
One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin-1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A-containing region. Thus, Sema3A and its co-receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.
References
SP1221 Pan, H. et al. (2009) Breast Can Res Treat. 118(1):197. WB: MDA-MB-231, shRNA knockdown
NP2111 Drenberg, C.D. et al. (2009) Obstet Gynecol Intl. 2009:1-8. IHC: human ovarian tissues
NP2111 Saban, M.R. et al. (2008) AJP Renal Phys. 295(1):F60. ICC: human urothelium
SP1221 Tang, X-Q. et al. (2007) J. Neurosci. 27(22):6068. WB: rat E14 spinal cord

Western blot image of COS-7 cells untransfected (lanes 1 & 3) or transfected with HA-tagged mouse NRP1 (lanes 2 & 4). Blots were probed with anti-NRP1 (NP2111) (lanes 1 & 2) or with anti-HA (lanes 3 & 4).

Formalin fixed, citric acid treated parafin sections of adult Rat cerebellum. Sections were probed with anti-Sema3A (SP1221) then anti-Rabbit:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).



Rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

NP2111 Boro, A. et al. (2015) Am J Transl Res. 7(3):640-653 WB: human osteosarcoma cell lines
PP1301 Cheadle, L. et al. (2014) J of Neuroscience 34(23): 7999. WB: rat brain
NP2111 Ohkusu-Tsukada, K. et al. (2010) Int J Cancer. Epub 15 MAR. ELISA: sNRP1 mouse serum
SP1221 Pan, H. et al. (2009) Breast Can Res Treat. 118(1):197. WB: MDA-MB-231, shRNA knockdown
NP2111 Drenberg, C.D. et al. (2009) Obstet Gynecol Intl. 2009:1-8. IHC: human ovarian tissues
NP2111 Saban, M.R. et al. (2008) AJP Renal Phys. 295(1):F60. ICC: human urothelium
SP1221 Tang, X-Q. et al. (2007) J. Neurosci. 27(22):6068. WB: rat E14 spinal cord

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
Neuropilin-1 (a1 CUB domain) Rabbit pAb50 μlWB, E, ICC, IHCHu, Rt, Ms130
Plexin A1 (Sema domain) Rabbit pAb50 μlWB, E, IP, ICC, IHCHu, Rt, Ms220
Plexin A1 (Sema domain) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms220
Semaphorin-3A (Central region) Rabbit pAb50 μlWB, E, ICC, IHCHu, Rt, Ms95
Semaphorin-3A (C-terminal) Rabbit pAb50 μlWB, EHu95
Semaphorin-3A (N-terminal) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms95
KIT SUMMARY

The Sema-3A and NRP1/Plexin A1 Receptor antibody sampler kit can be used to examine expression levels of Semaphorin-3A (Sema-3A) and it its co-receptors Neuropilin 1 (NRP1) and Plexin A1 in various species and applications.