Catalog # SK6030

Stat Phospho-Regulation Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395
The Stat family of proteins (Stat1-6) function both as cytoplasmic signal transducers and as activators of transcription in response to cytokines and growth factor receptors. Stat1 is expressed as two variants of 84 and 91 kDa that become phosphorylated at Tyr-701 in response to cytokines. Active Stat1 assembles into the interferon-stimulated gene factor 3 complex. Stat3 is expressed as two variants, Stat3α (92 kDa) and Stat3β (79 kDa) that differ in expression and activity. Both are activated by phosphorylation at Tyr-705, which induces dimerization, nuclear translocation and DNA binding. Stat3α transcriptional activation may be regulated by phosphorylation at Ser-727 through the MAPK pathway, while Stat3β lacks this serine site. Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr-694 is required for Stat5A activation and this Stat is constitutively active in some leukemic cell types. Both Stat5A (Tyr-694) and Stat5B (Tyr-699) are independently regulated and activated in various cell types.
References
Wen, Z. et al. (1995) Cell 82:241.
Meinke, A. et al. (1996) Mol. Cell. Biol. 16:6937-6944.
Darnell, J.E. (1997) Science 277:1630.
Biethahn, S. et al. (1999) Exp. Hematol. 27:885.

Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7 & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8 & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).

Immunocytochemical labeling of Stat5 in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal Stat5 (SM2511) or Stat5 (Tyr-694) (SM1481) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.



Mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
Stat1 Mouse mAb50 μlWB, E, IP, ICCHu, Rt, Ms91
Stat1 (Tyr-701), phospho-specific Mouse mAb50 μlWB, EHu, Rt, Ms91
Stat3 (N-terminal region) Mouse mAb50 μlWB, E, IPHu, Rt, Ms86
Stat5 (C-terminal region) Mouse mAb50 μlWB, E, ICCHu, Rt, Ms92
Stat5 (Tyr-694), phospho-specific Mouse mAb50 μlWB, E, ICCHu, Rt, Ms92
KIT SUMMARY

The Stat phospho-regulation antibody sampler kit can be used to examine the level of phosphorylation of Stat1 and Stat5, as well as the total expression level of Stat1, Stat3, and Stat5.