The Robo family of repulsive guidance receptors (Robo1-4) has important roles in controlling axon guidance and cell migration. These receptors are members of the immunoglobulin (Ig) superfamily and consist of an ectodomain with five Ig domains and three fibronectin type III repeats, a single transmembrane domain, and a long cytoplasmic tail that contains four blocks of conserved cytoplasmic sequences. In Drosophila, mutations in Robo, and its midline-expressed ligand Slit, result in too many axons crossing and staying at the midline. Several proteins that regulate the actin cytoskeleton, including cAbl, Ena, and Rho-family GTPases, contribute to the Robo signaling pathway. cAbl phosphorylates Robo1 at Tyr-1073, and this may inhibit Robo activity, while Slit-Robo signaling activates both Rac and Rho, and inactivates Cdc42. Thus, Robo guidance receptors control axon outgrowth and cell migration through activation of cell signaling pathways that regulate cytoskeletal dynamics.
References
Bashaw, G.J. et al. (2000). Cell 101:703.
Yang, L. & Bashaw, G.J. (2006). Neuron 52:595.
Western blot analysis of HepG2 (lanes 1 & 4), C2C12 (lanes 2 & 5), and HUVEC (lanes 3 & 6). The blot was probed with anti-Robo1 (C-terminal region) in absence (lanes 1-3) or presence of Robo1 (C-terminal region) blocking peptide (RX2795; lanes 4-6).
Western blot analysis of C2C12 and SYF mouse cell lines. The blots were probed with anti-Robo2 (C-terminal region) in the absence (-) or presence (+) of Robo2 (C-terminal region) blocking peptide (RX2865).
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| Robo1 (C-terminal region) Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 180 | |
| Robo1 (C-terminal region) Peptide | 50 μg | AB, E | |||
| Robo2 (C-terminal region) Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms, Ck | 175 | |
| Robo2 (C-terminal region) Peptide | 50 μg | AB, E | |||
| Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb | 100 μl | WB, E, ICC, IHC | Rb |
KIT SUMMARY
The Robo antibody sampler kit can be used to detect the level of Robo1 and Robo2 expression. The kit also includes peptides for antibody blocking experiments and a secondary reagent for antibody detection.
