Catalog # RK6080

Raf Phospho-Regulation Antibody Kit

Antibody Sampler Kit

Application / Dilution
ELISA, WB

Species Reactivity

Hu, Rt, Ms

$395


DATASHEET  

KIT SUMMARY

The Raf phospho-regulation antibody sampler kit can be used to examine phosphorylation of A-Raf, B-Raf, and C-Raf. The anti-B-Raf (Ser-446) detects site-specific phosphorylation of B-Raf, as well as the conserved site in C-Raf (Ser-338) and A-Raf (Ser-299). Similarly, the anti-C-Raf (Ser-471) detects site-specific phosphorylation in C-Raf, as well as in B-Raf (Ser-579) and A-Raf (Ser-432). The kit also includes antibodies to monitor total expression levels of A-Raf, B-Raf, and C-Raf.

Western blot of human Jurkat cells treated with calyculin A (100 nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated (lanes 2 & 4) with lambda phosphatase and probed with anti-C-Raf (N-terminal region) (lanes 1 & 2) or anti-C-Raf (Ser-471) (lanes 3 & 4).

Western blot of human Jurkat cells treated with calyculin A (100 nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated (lanes 2 & 4) with lambda phosphatase and probed with anti-B-Raf (N-terminus) (lanes 1 & 2) or anti-B-Raf (Ser-446) (lanes 3 & 4).



Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

CATALOG #DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
A-Raf (N-terminal region) Mouse mAb50 μlWB, EHu, Rt, Ms68
B-Raf (N-terminus) Rabbit pAb50 μlWB, EHu, Rt, Ms95
B-Raf (S446)[C-Raf (S338)/A-Raf (S299)], phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms95
C-Raf (C-terminus) Rabbit pAb50 μlWB, EHu, Rt, Ms74
C-Raf (N-terminal region) Mouse mAb50 μlWB, EHu, Rt, Ms74
C-Raf (S471)[B-Raf (S579)/A-Raf (S432)], phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms74