Catalog # PK7690

p38 MAPK Phospho-Regulation Staining Kit

Immunocytochemistry Kit

DATASHEET  




$405

p38 MAP kinase (MAPK), also called RK, CSBP, and SAPK2a, is the mammalian orthologue of the yeast HOG kinase. This family of kinases participates in signaling cascades that control cellular responses to cytokines and stress. Four isoforms of p38 MAPK (α,β,γ,δ) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides, UV light, and growth factors. MKK3 and SEK activate p38 MAPK by dual phosphorylation at Thr-180/Tyr-182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2, Max, and MEF2. T cells possess an alternate pathway for p38 activation where stimulation of the antigen receptor (TCR) induces phosphorylation of p38 on Tyr-323. This site is required for TCR-mediated phosphorylation of Thr-180 and catalytic activity. Thus, Tyr-323 may also have important roles in regulating p38 MAP kinase pathways.

References
Freshney, N. W. et al. (1994) Cell 78:1039.
Raingeaud, J. et al. (1995) J. Biol. Chem. 270:7420.
Salvador, J.M. et al. (2005) Nat Immunol. 6(4):390.
Jirmanova, L. et al. (2009) Blood 113(10):2229.

Immunocytochemical labeling of p38 MAPK in control and pervanadate-treated mouse C2C12 cells. The cells were labeled with mouse monoclonal p38α MAPK (PM1381), p38 MAPK (Thr-180/Tyr-182) (PM1391), and rabbit polyclonal p38 MAPK (Tyr-323) (PP3411). The antibodies were detected using appropriate secondary conjugated to DyLight® 488 or DyLight® 594.



Mouse monoclonal, rabbit polyclonal, and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

PP3411 Alam, M.S. et al. (2015) Nat Medicine 21:1337. WB, IF: human T-cells, CD3 activated
PP3411 Lanna, A. et al. (2014) Nat Immunol. 15(10):965. WB: human T-cells, CD3 activated
PM1391 Chambers, M.A. et al. (2009) J Physiol. 587:3363. WB: mouse muscle, C2C12
PM1391 Li, W. et al. (2009) AJP Cell Phys. 297:C706. WB: mouse C2C12 cells

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYICC DILUTION
p38α MAP Kinase (C-terminal) Mouse mAb50 μlWB, E, ICCHu, Rt, Ms1:200
p38 MAP Kinase (Thr-180/Tyr-182), phospho-specific Mouse mAb50 μlWB, E, ICCHu, Rt, Ms 1:250
p38α MAP Kinase (Tyr-323), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck 1:300
Anti-Mouse Ig:DyLight® 488 Goat pAb100 μlICC, IHCMs1:200
Anti-Rabbit Ig:DyLight® 594 Goat pAb100 μlICC, IHCRb1:200
KIT SUMMARY

The p38 MAPK phospho-regulation kit can be used for immunocytochemical co-localization of p38 MAPK phosphorylation at Thr-180/Tyr-182 and Tyr-323 relative to the total expression of p38 MAPK. The kit includes rabbit polyclonal and mouse monoclonal antibodies along with Goat-anti-Rabbit conjugated to DyLight® 594 and Goat anti-Mouse conjugated to DyLight® 488 for dual labeling experiments.