Catalog # PK7660


Paxillin Phospho-Regulation

Immunocytochemistry Kit

$355

Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both ERK and p38MAPK kinases phosphorylate serine 83 in vitro. HGF stimulation of murine epithelial cells leads to ERK-mediated phosphorylation of Ser-83, which is required for HGF-induced cell spreading and migration. In addition, Ser-83 is phosphorylated in response to NGF in PC12 cells, and this phosphorylation may be involved in neurite extension. In human paxillin, Ser-85 rather than Ser-83 may be the site phosphorylated by p38 MAPK and mutation of this site inhibits NGF-induced neurite extension. Thus, serine residues in the N-terminal region of paxillin may be important for growth-factor mediated changes in activity.

References
Woodrow, M.A. (2003) Exp. Cell. Res. 287(2):325-338.
Huang, C. et al. (2004) J Cell Biol. 164(4):593-602.
Huang, C. et al. (2004) Cell Cycle 3(1):4-6.
Ishibe, S. et al. (2004) Mol. Cell 16 :257-267.
Mouse monoclonal, rabbit polyclonal, and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

PM1071 Omori, K. et al. (2015) Bioch Biophys Res Com 458(4): 934. IF: mouse NIH3T3 fibroblasts
PP1341 Wu, D. W. et al. (2014) Oncogene 33(35): 4385. WB: CCM3 cells
PM1071 Cheng, I. et al. (2014) J Biol Chem. 289(39):26989 IF: B16-F1 cells
PM1071 Liu, H. et al. (2013) BMC Cancer. 13:80. WB: human ovarian cancer cells
PM1071 Lee, S. et al. (2013) Biochim Biophys Acta. 1830(8):4017. WB: rat C6 and human U373 gliomas
PP1341 Sen, A. et al. (2012) J Clin Invest. 122(7):2469. WB, ICC: C4C2, PC3, LNCaP
PP1341 Kwak, T.K. et al. (2010) J Biol Chem. 285(46):36021. WB: pancreatic INS-1 cells
PP1341 Sen, A. et al. (2010) J Biol Chem. 285(37):28787. WB: human LNCAP cells

Immunocytochemical labeling of phosphorylated paxillin in paraformaldehyde fixed and NP-40 permeabilized rat A7r5 cells. The cells were labeled with mouse monoclonal Paxillin (PM1071; left) and rabbit polyclonal Paxillin (Ser-83) (PP1341, right). The antibodies were detected with Goat anti-Mouse DyLight® 488 and Goat anti-Rabbit DyLight® 594, respectively.



This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYICC DILUTION
Paxillin Mouse mAb50 μlWB, E, IP, ICCHu, Rt, Ms, Ck1:100
Paxillin (Ser-83), phospho-specific Rabbit pAb50 μlWB, E, ICCRt, Ms 1:50
Anti-Mouse Ig:DyLight® 488 Goat pAb100 μlICC, IHCMs1:200
Anti-Rabbit Ig:DyLight® 594 Goat pAb100 μlICC, IHCRb1:200
KIT SUMMARY

The paxillin phospho-regulation kit can be used for immunocytochemical co-localization of paxillin phosphorylation at Ser-83 compared to the total expression of paxillin. The kit includes rabbit polyclonal and mouse monoclonal antibodies along with Goat-anti-rabbit conjugated to DyLight® 594 and Goat anti-mouse conjugated to DyLight® 488 for dual labeling experiments.