Catalog # PK7660

Paxillin Phospho-Regulation Staining Kit

Immunocytochemistry Kit

Application / Dilution
ELISA, WB, ICC, IHC, IP

Species Reactivity

Hu, Rt, Ms, Ck, Rb

$355


DATASHEET  

KIT SUMMARY

The paxillin phospho-regulation kit can be used for immunocytochemical co-localization of paxillin phosphorylation at Ser-83 compared to the total expression of paxillin. The kit includes rabbit polyclonal and mouse monoclonal antibodies along with Goat-anti-rabbit conjugated to DyLight® 594 and Goat anti-mouse conjugated to DyLight® 488 for dual labeling experiments.

Immunocytochemical labeling of phosphorylated paxillin in paraformaldehyde fixed and NP-40 permeabilized rat A7r5 cells. The cells were labeled with mouse monoclonal Paxillin (PM1071; left) and rabbit polyclonal Paxillin (Ser-83) (PP1341, right). The antibodies were detected with Goat anti-Mouse DyLight® 488 and Goat anti-Rabbit DyLight® 594, respectively.



Mouse monoclonal, rabbit polyclonal, and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

PM1071 Omori, K. et al. (2015) Bioch Biophys Res Com 458(4): 934. IF: mouse NIH3T3 fibroblasts
PP1341 Wu, D. W. et al. (2014) Oncogene 33(35): 4385. WB: CCM3 cells
PM1071 Cheng, I. et al. (2014) J Biol Chem. 289(39):26989 IF: B16-F1 cells
PM1071 Liu, H. et al. (2013) BMC Cancer. 13:80. WB: human ovarian cancer cells
PM1071 Lee, S. et al. (2013) Biochim Biophys Acta. 1830(8):4017. WB: rat C6 and human U373 gliomas
PP1341 Sen, A. et al. (2012) J Clin Invest. 122(7):2469. WB, ICC: C4C2, PC3, LNCaP
PP1341 Kwak, T.K. et al. (2010) J Biol Chem. 285(46):36021. WB: pancreatic INS-1 cells
PP1341 Sen, A. et al. (2010) J Biol Chem. 285(37):28787. WB: human LNCAP cells

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYICC DILUTION
Paxillin Mouse mAb50 μlWB, E, IP, ICCHu, Rt, Ms, Ck1:100
Paxillin (Ser-83), phospho-specific Rabbit pAb50 μlWB, E, ICCRt, Ms 1:50
Anti-Mouse Ig:DyLight® 488 Goat pAb100 μlICC, IHCMs1:200
Anti-Rabbit Ig:DyLight® 594 Goat pAb100 μlICC, IHCRb1:200