Catalog # PK7660
Paxillin Phospho-Regulation Staining Kit
Immunocytochemistry Kit
| ELISA, WB, ICC, IHC, IP |
Species Reactivity
| Hu, Rt, Ms, Ck, Rb |
 |
KIT SUMMARY The paxillin phospho-regulation kit can be used for immunocytochemical co-localization of paxillin phosphorylation at Ser-83 compared to the total expression of paxillin. The kit includes rabbit polyclonal and mouse monoclonal antibodies along with Goat-anti-rabbit conjugated to DyLight® 594 and Goat anti-mouse conjugated to DyLight® 488 for dual labeling experiments. |
Immunocytochemical labeling of phosphorylated paxillin in paraformaldehyde fixed and NP-40 permeabilized rat A7r5 cells. The cells were labeled with mouse monoclonal Paxillin (PM1071; left) and rabbit polyclonal Paxillin (Ser-83) (PP1341, right). The antibodies were detected with Goat anti-Mouse DyLight® 488 and Goat anti-Rabbit DyLight® 594, respectively.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
PM1071 Omori, K. et al. (2015) Bioch Biophys Res Com 458(4): 934. IF: mouse NIH3T3 fibroblastsPP1341 Wu, D. W. et al. (2014) Oncogene 33(35): 4385. WB: CCM3 cells
PM1071 Cheng, I. et al. (2014) J Biol Chem. 289(39):26989 IF: B16-F1 cells
PM1071 Liu, H. et al. (2013) BMC Cancer. 13:80. WB: human ovarian cancer cells
PM1071 Lee, S. et al. (2013) Biochim Biophys Acta. 1830(8):4017. WB: rat C6 and human U373 gliomas
PP1341 Sen, A. et al. (2012) J Clin Invest. 122(7):2469. WB, ICC: C4C2, PC3, LNCaP
PP1341 Kwak, T.K. et al. (2010) J Biol Chem. 285(46):36021. WB: pancreatic INS-1 cells
PP1341 Sen, A. et al. (2010) J Biol Chem. 285(37):28787. WB: human LNCAP cells
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | ICC DILUTION |
| Paxillin Mouse mAb | 50 μl | WB, E, IP, ICC | Hu, Rt, Ms, Ck | 1:100 | |
| Paxillin (Ser-83), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Rt, Ms | 1:50 | |
| Anti-Mouse Ig:DyLight® 488 Goat pAb | 100 μl | ICC, IHC | Ms | 1:200 | |
| Anti-Rabbit Ig:DyLight® 594 Goat pAb | 100 μl | ICC, IHC | Rb | 1:200 |
