Catalog # PK6930

Profilin Phospho-Regulation Antibody Kit

Antibody Sampler Kit

Application / Dilution
ELISA, WB, ICC, IHC

Species Reactivity

Hu, Rt, Ms, Rb

$395


DATASHEET  

KIT SUMMARY

The Profilin phospho-regulation antibody sampler kit can be used to detect phosphorylation of Tyr-129 and Ser-138 relative to total profilin expression levels. The kit includes rabbit polyclonal phospho-specific antibodies to Tyr-129 and Ser-138, as well as rabbit polyclonal antibodies that detect different epitopes in the C-terminal region of Profilin. The kit also includes an anti-Rabbit Light Chain specific:HRP secondary reagent for detection of rabbit polyclonal antibody in western blot, ELISA, or immunocytochemistry.

Western blot of HUVEC stimulated with Pervanadate (1 mM) for 30 min (lanes 1-4). The blots were treated with alkaline phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Profilin (a.a. 126-137) (lanes 1 & 2) or anti-Profilin (Tyr-129) phospho-specific (lanes 3 & 4). The antibodies detect profilin at 15 kDa. In lanes 3 & 4, the antibody also detects an unknown 20 kDa protein.

Western blot of Jurkat stimulated with calyculin A (100 nM) for 30 min (lanes 1-6). The blots were probed with anti-Profilin (a.a. 132-140) (lanes 1-3) or anti-Profilin (Ser-138) phospho-specific (lanes 4-6). Both antibodies were used in the absence (lanes 1 & 4) or presence of unphosphorylated Profilin (Ser-138) (lanes 2 & 5) or phospho-Profilin (Ser-138) (lanes 3 & 6) blocking peptides.



Rabbit polyclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

PP4821 Stanslowsky N. et al. (2016) J Neurosci. 36(47):12027. (WB: human striatal medium spiny neurons)
PP4791 Stanslowsky N. et al. (2016) J Neurosci. 36(47):12027. (WB: human striatal medium spiny neurons)
PP4751 Dombert B. et al. (2017) Front Mol Neurosci. 10:346. (ICC: mouse spinal motoneurons)
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. (WB, ICC: mouse intestinal myofibroblasts)
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. (Western Blot)

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
Profilin (a.a. 132-140) Rabbit pAb50 μlWB, EHu, Rt, Ms15
Profilin (Ser-138), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms15
Profilin (a.a. 126-137) Rabbit pAb 50 μlWB, EHu, Rt, Ms15
Profilin (Tyr-129), phospho-specific Rabbit pAb 50 μlWB, EHu, Rt, Ms15
Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb100 μlWB, E, ICC, IHCRb