Catalog # PK6650
Paxillin Phospho-Tyrosine Regulation Antibody Kit
Antibody Sampler Kit
| ELISA, WB, IP, ICC, IHC |
Species Reactivity
| Hu, Rt, Ms, Ck, F |
 |
KIT SUMMARY The paxillin phospho-tyrosine regulation antibody kit can be used to detect phosphorylation of paxillin at Tyr-31 and Tyr-118 relative to total paxillin expression. The kit includes mouse monoclonal and rabbit polyclonal antibodies to detect paxillin expression, as well as secondary reagents for detection in western blot. |
Western blot analysis of A431 cells (30 µg/lane) serum starved overnight (lane 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Paxillin (PM1071) (lanes 1 & 2) and anti-phospho-Paxillin (Tyr-31) (PM1021) (lanes 3 & 4).
Western blot analysis of A431 cells (30 mg/lane) serum starved overnight (lane 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Paxillin (PM1071) (lanes 1 & 2) and rabbit polyclonal anti-phospho-Paxillin (Tyr-118) (PP4501) (lanes 3 & 4).
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
PM1071 Omori, K. et al. (2015) Bioch Biophys Res Com 458(4): 934. IF: mouse NIH3T3 fibroblastsPM1071 Cheng, I. et al. (2014) J Biol Chem. 289(39):26989 IF: B16-F1 cells
PM1021 Lee, S. et al. (2013) Biochim Biophys Acta. 1830(8):4017. WB: rat C6 and human U373 gliomas
PM1071 Lee, S. et al. (2013) Biochim Biophys Acta. 1830(8):4017. WB: rat C6 and human U373 gliomas
PM1071 Liu, H. et al. (2013) BMC Cancer. 13:80. WB: human ovarian cancer cells
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
PM1021 Zou, W. et al. (2012) J Bone Miner Res. 27(12):2490. WB: mouse bone marrow macrophages
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| Paxillin Mouse mAb | 50 μl | WB, E, IP, ICC | Hu, Rt, Ms, Ck | 68 | |
| Paxillin (C-terminal) Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 68 | |
| Paxillin (Tyr-31), phospho-specific Mouse mAb | 50 μl | WB, E | Hu, Rt, Ms | 68 | |
| Paxillin (Tyr-118), phospho-specific Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms, Ck, F | 68 | |
| Anti-Mouse Ig:HRP Donkey pAb | 100 μl | WB, E | Ms | ||
| Anti-Rabbit Ig Light ChainSpecific:HRP Mouse mAb | 100 μl | WB, E, ICC, IHC | Rb |
