Catalog # PK6650

Paxillin Phospho-Tyrosine Regulation Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395

Paxillin is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and an SH3- and SH2-binding domain that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Tyrosine phosphorylation of paxillin occurs in response to growth factors, neuropeptides, and integrins. The major sites of tyrosine phosphorylation include Tyr-31 and Tyr-118. Both of these sites may be involved in Crk binding to paxillin during integrin-mediated cell adhesion. These sites may provide docking motifs for recruitment of other signaling molecules to focal adhesions.

References
Schaller, M.D & Schaefer, E.M. (2001) Biochem J. 360:57.
Huang, C. et al. (2003) Nature 424:219-223.
Woodrow, M.A. (2003) Exp. Cell. Res. 287(2):325-338.
Huang, C. et al. (2004) Cell Cycle 3(1):4-6.

Western blot analysis of A431 cells (30 µg/lane) serum starved overnight (lane 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Paxillin (PM1071) (lanes 1 & 2) and anti-phospho-Paxillin (Tyr-31) (PM1021) (lanes 3 & 4).

Western blot analysis of A431 cells (30 mg/lane) serum starved overnight (lane 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Paxillin (PM1071) (lanes 1 & 2) and rabbit polyclonal anti-phospho-Paxillin (Tyr-118) (PP4501) (lanes 3 & 4).



Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

PM1071 Omori, K. et al. (2015) Bioch Biophys Res Com 458(4): 934. IF: mouse NIH3T3 fibroblasts
PM1071 Cheng, I. et al. (2014) J Biol Chem. 289(39):26989 IF: B16-F1 cells
PM1021 Lee, S. et al. (2013) Biochim Biophys Acta. 1830(8):4017. WB: rat C6 and human U373 gliomas
PM1071 Lee, S. et al. (2013) Biochim Biophys Acta. 1830(8):4017. WB: rat C6 and human U373 gliomas
PM1071 Liu, H. et al. (2013) BMC Cancer. 13:80. WB: human ovarian cancer cells
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
PM1021 Zou, W. et al. (2012) J Bone Miner Res. 27(12):2490. WB: mouse bone marrow macrophages
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
Paxillin Mouse mAb50 μlWB, E, IP, ICCHu, Rt, Ms, Ck68
Paxillin (C-terminal) Rabbit pAb50 μlWB, EHu, Rt, Ms68
Paxillin (Tyr-31), phospho-specific Mouse mAb50 μlWB, EHu, Rt, Ms68
Paxillin (Tyr-118), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms, Ck, F68
Anti-Mouse Ig:HRP Donkey pAb 100 μlWB, EMs
Anti-Rabbit Ig Light ChainSpecific:HRP Mouse mAb 100 μlWB, E, ICC, IHCRb
KIT SUMMARY

The paxillin phospho-tyrosine regulation antibody kit can be used to detect phosphorylation of paxillin at Tyr-31 and Tyr-118 relative to total paxillin expression. The kit includes mouse monoclonal and rabbit polyclonal antibodies to detect paxillin expression, as well as secondary reagents for detection in western blot.