Catalog # PK6620

Paxillin Phospho-Ser/Thr Regulation Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395

Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at tyrosine, threonine, and serine sites. Serine and threonine phosphorylation of paxillin occur in response to growth-factor activation, PKC activators, and fibronectins. Phosphorylation of Ser-85, Ser-178, and Thr-538 may be important sites for regulating paxillin activity. Paxillin phosphorylation of Thr-538 occurs in response to TPA-activated PKCs in vitro, and this phosphorylation may contribute to dissolution of the actin cytoskeleton and redistribution of LFA-1 integrins in vivo.

References
Huang, C. et al. (2004) Cell Cycle 3(1):4-6.
Ishibe, S. et al. (2004) Mol. Cell 16 :257-267.
Huang, C. et al. (2004) J Cell Biol. 164(4):593-602.
Romanova, L.Y. et al. (2010) J Cell Sci. 123:1567-1577.

Western blot analysis of PC12 cells untreated (lanes 1 & 3) or treated with NGF (200 ng/ml) for 60 min (lanes 2 & 4). The blots were probed with mouse monoclonal anti-Paxillin (PM1071; lane 1 & 2) or rabbit polyclonal anti-Paxillin (Ser-83) phospho-specific antibody (lane 3 & 4).

Western blot analysis of human A431 cells treated with Calyculin A (100 nm) for 30 min (lanes 1-6). The blot was treated with lambda phosphatase (lanes 2, 4, & 6), then probed with rabbit polyclonal anti-Paxillin (C-terminal; PP1161) (lanes 1 & 2), anti-phospho-Paxillin (Thr-538; PP4491) (lanes 3 & 4), and mouse monoclonal anti-Paxillin (PM1071) (lanes 5 & 6).



Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

PM1071 Omori, K. et al. (2015) Bioch Biophys Res Com 458(4): 934. IF: mouse NIH3T3 fibroblasts
PP1051 Wu, D. W. et al. (2014) Oncogene 33(35): 4385. WB: CCM3 cells
PP1341 Wu, D. W. et al. (2014) Oncogene 33(35): 4385. WB: CCM3 cells
PP4491 Wu, D. W. et al. (2014) Oncogene 33(35): 4385. WB: CCM3 cells
PM1071 Cheng, I. et al. (2014) J Biol Chem. 289(39):26989 IF: B16-F1 cells
PP1051 Liu, H. et al. (2013) BMC Cancer. 13:80. WB: human ovarian cancer cells
PP1051 Wei, W. et al. (2013) Pancreatology 13(4):384. WB: pancreatic PANC1, Capan-2 and HT1080 cells
PM1071 Lee, S. et al. (2013) Biochim Biophys Acta. 1830(8):4017. WB: rat C6 and human U373 gliomas
PM1071 Liu, H. et al. (2013) BMC Cancer. 13:80. WB: human ovarian cancer cells
PP1341 Sen, A. et al. (2012) J Clin Invest. 122(7):2469. WB, ICC: C4C2, PC3, LNCaP
PP1341 Kwak, T.K. et al. (2010) J Biol Chem. 285(46):36021. WB: pancreatic INS-1 cells
PP1341 Sen, A. et al. (2010) J Biol Chem. 285(37):28787. WB: human LNCAP cells
PP1051 Kwak, T.K. et al. (2010) J Biol Chem. 285(46):36021. WB: pancreatic INS-1 cells

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
Paxillin Mouse mAb50 μlWB, E, IP, ICCHu, Rt, Ms, Ck68
Paxillin (C-terminal) Rabbit pAb50 μlWB, EHu, Rt, Ms68
Paxillin (Ser-83), phospho-specific Rabbit pAb50 μlWB, E, ICCRt, Ms68
Paxillin (Ser-178), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms68
Paxillin (Thr-538), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms, Ck, F68
KIT SUMMARY

The paxillin phospho-ser/thr regulation antibody kit can be used to detect phosphorylation of paxillin at Ser-83, Ser-178, and Thr-538 relative to total paxillin expression. The kit includes mouse monoclonal and rabbit polyclonal antibodies to detect paxillin expression, as well as secondary reagents for detection in western blot.