Catalog # PK6580


Plexin A1, B1, and D1 Receptors

Antibody Sampler Kit

$395

Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. Plexin A1 and its co-receptor Neuropilin 1 bind Sema-3A leading to collapse of neuronal axon growth cones. The expression of Plexin-A1 and its co-receptor is upregulated in neurons after central nervous system injury, and the Sema-3A activation of Plexin-A1 and Neuropilin-1 co-receptors may have significant roles in suppressing axon regeneration after neuronal injury. Plexin-B1 is expressed as a 300 kDa precursor protein that can be cleaved into a 200 kDa a-subunit and a 100 kDa b-subunit. This plexin is the receptor for the class IV semaphorin, Sema-4D, and the Sema-4D/Plexin B1 pathway may be involved in cell survival, cell migration, and angiogenesis. Plexin D1 is a type I transmembrane protein that may be expressed as multiple isoforms in many cell types, including neurons and endothelial cells. Semaphorin 3E (Sema-3E) and semaphorin 4A can bind Plexin D1 leading to phosphorylation of Tyr-1642 and activation of Plexin D1. The Sema3E/Plexin D1 pathway may have important roles in angiogenesis and metastasis.

References
Casazza, A. et al. (2010) J Clin Invest. 120(8):2684.
Basile, J.R. et al. (2006) PNAS 103(24):9017.
Takahashi, T. & Strittmatter, S.M. (2001) Neuron 29:429.
Tamagnone, L. et al. (1999) Cell 99:71.
Rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

PP1301 Cheadle, L. et al. (2014) J of Neuroscience 34(23): 7999. (WB: rat brain)
PP1841 Damola, A. et al. (2013) Prostate 73(12):1326. (WB, IP: Prostate Cells, shRNA knockdown)
PP1841 Soong, J. & Scott, G. (2013) J Cell Sci. 126(Pt 2):688. (WB: human melanocytes)
PP1841 Soong, J. et al (2012) J Invest Dermatol. 132(4):1230. (WB: melanocyte, keratinocyte, smooth muscle)

Western blot analysis of Plexin D1 expression in human endothelial cells (HUVEC) (lanes 1-6). The blots were probed with rabbit polyclonals anti-Plexin D1 (Sema domain) (lanes 1 & 2), anti-Plexin D1 (Cytoplasmic domain) (lanes 3 & 4), and anti-Plexin D1 (a.a. 1635-1647) (lanes 5 & 6). Each antibody was used in the presence of their respective blocking peptide (lanes 2, 4 & 6).

Western blots showing Cos7 cells transfected with Myc-tagged Plexin A1 (lanes 1 & 3), neonatal rat brain (lane 2), or Plexin-A1 immunoprecipitated from transfected cells using anti-Myc (lane 4) or anti-Plexin-A1 (PP1301; lane 5). These blots were probed with anti-Plexin-A1 (PP1301; lanes 1 & 2) or with anti-Myc (lanes 3-5).



This kit contains:

CATALOG #DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
Plexin A1 (Sema Domain) Rabbit pAb50 μlWB, E, IP, ICC, IHCHu, Rt, Ms220
Plexin A1 (Sema Domain) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms220
Plexin B1 (C-terminal region) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms300
Plexin D1 Cytoplasmic domain) Rabbit pAb50 μlWB, EHu, Rt, Ms250
Plexin D1 (Sema domain) Rabbit pAb50 μlWB, EHu, Rt, Ms250
Plexin D1 (a.a. 1635-1647) Rabbit pAb50 μlWB, EHu, Rt, Ms250
KIT SUMMARY

The Plexin Receptor kit can be used to monitor the expression of Plexin A1, Plexin B1, and Plexin D1. The kit includes three antibodies for detecting different regions of Plexin D1, two antibodies to different regions in the Sema domain of Plexin A1, and an antibody that detects the C-terminal region of Plexin B1.