Catalog # PK6540

PKC (α,β,γ) Phospho-Regulation Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395

The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (α, β1, βII, and γ) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. The phosphorylation of multiple sites in conventional PKCs regulates their activity. In mast cells, FceRI stimulation leads to phosphorylation of tyrosine 658 and 662 of PKCα and PKCβI respectively. This phosphorylation requires autophosphorylation of serine 657 and 661 in these respective kinases.

References
Nishizuka, Y. (1988) Nature 334:661.
Thiels, E. et al. (2000) J Neurosci. 20(19):7199.
Kawakami et al. (2003) PNAS. USA 100:9470-9475.

Western blot analysis of immunoprecipitates from neonatal rat brain lysate using anti-PKCα antibody. Control and alkaline phosphatase treated precipitates were probed with anti-PKCα (Central region) or anti-phospho-PKCα (Ser-657/Tyr-658). The latter shows no detection of PKCα after phosphatase treatment.

Formalin fixed, citric acid treated parafin sections of adult mouse brain. Sections were probed with anti-PKCα (PM2371) then anti-mouse:HRP before detection using DAB. (Image provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).



Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
PKCα(Central region) Mouse mAb50 μlWB, E, IP, ICC, IHCHu, Rt, Ms82
PKC (α,β,γ) Mouse mAb50 μlWB, EHu, Rt, Ms, Ck82
PKCα(Ser-657/Tyr-658), phospho-specific Rabbit pAb 50 μlWB, E, IP, ICCHu, Rt, Rb82
Anti-Mouse Ig:HRP Donkey pAb100 μlWB, EMs
Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb100 μlWB, E, ICC, IHCRb
KIT SUMMARY

The PKC (α,β,γ) phospho-regulation kit can be used to examine phosphorylation of PKCα (Ser-657/Tyr-658) relative to the total expression of PKCα. The phospho-specific antibody also detects phosphorylation of the the conserved dual site in PKCβ and PKCγ. The kit includes mouse monoclonal antibodies to detect total PKCα and PKC (α,β,γ), as well as secondary reagents for rabbit polyclonal and mouse monoclonal antibody detection.