Catalog # PK6400

PDK1 Phospho-Regulation Antibody Kit

Antibody Sampler Kit

Application / Dilution
ELISA, WB, ICC, IHC

Species Reactivity

Hu, Rt, Ms, Rb

$395


DATASHEET  

KIT SUMMARY

The PDK1 phospho-regulation antibody sampler kit can be used to examine phosphorylation of PDK1 at Ser-241 and Tyr-9. The kit also includes monoclonal and polyclonal antibodies to monitor total expression levels of PDK1, as well as secondary reagents for antibody detection.

Western blot of MDA-MB-435 cells untreated (lanes 1 and 3) or treated with EGF (100 ng/ml) for 1 hr (lanes 2 & 4). Blots were probed with mouse monoclonal anti-PDK1 (C-terminal region) or rabbit polyclonal anti-PDK1 (Ser-241).

Immunocytochemical labeling of PDK1 phosphorylation in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal PDK1 (C-terminal Region) (PM1461), rabbit polyclonal PDK1 (Tyr-9) (PP1431), and PDK1 (N-terminus) (PP1411) antibodies. These antibodies were detected using appropriate secondary antibody conjugated to DyLight 594.



Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
PM1461 Choucair, K.A. et al. (2012) Transl Oncol. 5(6):453. WB: LNCaP, PC-3, and DU145 PCa cell
PP1431 Mashukovaa, A. et al. (2012) Mol Biol Cell. 23(9):1664. WB: Caco-2 cells
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
PP1431 Dodson, L.F. et al. (2009) Mol Cell Biol. 29:3710. WB: mouse T-Cells
PP1431 Block, K. et al. (2008) J. Biol. Chem. 35:24061. WB: rat mesangial cells

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
PDK1 (N-terminus) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms65
PDK1 (C-terminal region) Mouse mAb50 μlWB, E, ICCHu, Rt, Ms65
PDK1 (Tyr-9), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms65
PDK1 (Ser-241), phospho-specific Rabbit pAb50 μlWB, E, IHCHu, Rt, Ms65
Anti-Mouse Ig:HRP Donkey pAb 100 μlWB, EMs
Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb 100 μlWB, E, ICC, IHCRb