Catalog # PK6400


PDK1 Phospho-Regulation

Antibody Sampler Kit

$395

3-Phosphoinositide-dependent kinase 1 (PDK1), also known as PKB kinase, was identified as the activator of the survival kinase Akt/PKB. Several important substrates of PDK1 include p70S6 kinase, PKAs, PKCs, SGKs, RSKs, and PAKs. PDK1 is a member of the AGC superfamily of serine/threonine kinases. Through the phosphorylation of downstream kinases like Akt, PDK1 has been shown to be involved in several different cell functions, such as protein synthesis, cell survival, glucose metabolism, and cell adhesion and migration. The regulation of PDK1 occurs through lipid second messengers and phosphorylation. Multiple serine sites are phosphorylated on PDK1. Serine 241 phosphorylation is required for PDK1 activity, while serine 396 has been implicated in PDK1 nuclear translocation. Tyrosine phosphorylation may also regulate PDK1 activity. Tyrosines 9 and 373/376 are phosphorylated by c-Src in vitro. Tyr-373/Tyr-376 is important for PDK1 activity, while Tyr-9 phosphorylation permits Tyr-373/Tyr-376 phosphorylation by c-Src. In addition, Tyr-9 may be important during angiotensin-II-induced focal adhesion formation.

References
Park, J. et al. (2001) J Biol. Chem. 276(40):37459.
Taniyama, Y. et al. (2003) Mol Cell. Biol. 23(22):8019.
Scheid, M.P. et al. (2005) Mol. Cell. Biol. 25(6):2347.
Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
PM1461 Choucair, K.A. et al. (2012) Transl Oncol. 5(6):453. WB: LNCaP, PC-3, and DU145 PCa cell
PP1431 Mashukovaa, A. et al. (2012) Mol Biol Cell. 23(9):1664. WB: Caco-2 cells
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
PP1431 Dodson, L.F. et al. (2009) Mol Cell Biol. 29:3710. WB: mouse T-Cells
PP1431 Block, K. et al. (2008) J. Biol. Chem. 35:24061. WB: rat mesangial cells

Western blot of MDA-MB-435 cells untreated (lanes 1 and 3) or treated with EGF (100 ng/ml) for 1 hr (lanes 2 & 4). Blots were probed with mouse monoclonal anti-PDK1 (C-terminal region) or rabbit polyclonal anti-PDK1 (Ser-241).

Immunocytochemical labeling of PDK1 phosphorylation in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal PDK1 (C-terminal Region) (PM1461), rabbit polyclonal PDK1 (Tyr-9) (PP1431), and PDK1 (N-terminus) (PP1411) antibodies. These antibodies were detected using appropriate secondary antibody conjugated to DyLight 594.



This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
PDK1 (N-terminus) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms65
PDK1 (C-terminal region) Mouse mAb50 μlWB, E, ICCHu, Rt, Ms65
PDK1 (Tyr-9), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms65
PDK1 (Ser-241), phospho-specific Rabbit pAb50 μlWB, E, IHCHu, Rt, Ms65
Anti-Mouse Ig:HRP Donkey pAb 100 μlWB, EMs
Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb 100 μlWB, E, ICC, IHCRb
KIT SUMMARY

The PDK1 phospho-regulation antibody sampler kit can be used to examine phosphorylation of PDK1 at Ser-241 and Tyr-9. The kit also includes monoclonal and polyclonal antibodies to monitor total expression levels of PDK1, as well as secondary reagents for antibody detection.