Catalog # PK6400
PDK1 Phospho-Regulation Antibody Kit
Antibody Sampler Kit
| ELISA, WB, ICC, IHC |
Species Reactivity
| Hu, Rt, Ms, Rb |
 |
KIT SUMMARY The PDK1 phospho-regulation antibody sampler kit can be used to examine phosphorylation of PDK1 at Ser-241 and Tyr-9. The kit also includes monoclonal and polyclonal antibodies to monitor total expression levels of PDK1, as well as secondary reagents for antibody detection. |
Western blot of MDA-MB-435 cells untreated (lanes 1 and 3) or treated with EGF (100 ng/ml) for 1 hr (lanes 2 & 4). Blots were probed with mouse monoclonal anti-PDK1 (C-terminal region) or rabbit polyclonal anti-PDK1 (Ser-241).
Immunocytochemical labeling of PDK1 phosphorylation in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal PDK1 (C-terminal Region) (PM1461), rabbit polyclonal PDK1 (Tyr-9) (PP1431), and PDK1 (N-terminus) (PP1411) antibodies. These antibodies were detected using appropriate secondary antibody conjugated to DyLight 594.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblastsPM1461 Choucair, K.A. et al. (2012) Transl Oncol. 5(6):453. WB: LNCaP, PC-3, and DU145 PCa cell
PP1431 Mashukovaa, A. et al. (2012) Mol Biol Cell. 23(9):1664. WB: Caco-2 cells
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
PP1431 Dodson, L.F. et al. (2009) Mol Cell Biol. 29:3710. WB: mouse T-Cells
PP1431 Block, K. et al. (2008) J. Biol. Chem. 35:24061. WB: rat mesangial cells
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| PDK1 (N-terminus) Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 65 | |
| PDK1 (C-terminal region) Mouse mAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 65 | |
| PDK1 (Tyr-9), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 65 | |
| PDK1 (Ser-241), phospho-specific Rabbit pAb | 50 μl | WB, E, IHC | Hu, Rt, Ms | 65 | |
| Anti-Mouse Ig:HRP Donkey pAb | 100 μl | WB, E | Ms | ||
| Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb | 100 μl | WB, E, ICC, IHC | Rb |
