Catalog # PK6330

Phospho-Tyrosine, Serine, Threonine Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395

Phosphorylation of specific tyrosine (tyr), serine (ser), or threonine (thr) residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr and tyr kinases that mediate these protein modifications. Antibodies that can detect phosphotyrosine, phosphoserine, or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest, followed by detection of tyr, ser, and thr phosphorylation using these antibodies is commonly used to correlate changes in phosphorylation state with alterations in protein activity.

References
Hunter T.(1987) Cell. 50(6):823.
Wang, J.Y.J. (1988) Anal. Biochem 172:1.
Krishna, R.G. & Wold, F. (1993) Adv Enzymol Rel Areas Mol Biol 67:265.
Yaffe, M.B. & Elia, A.E. (2001) Curr Opin Cell Biol 13, 131-8.

Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1) then treated with lambda phosphatase (lane 2). The blot was probed with anti-Phosphoserine/threonine rabbit polyclonal at 1:1000.

Immunocytochemical labeling of phosphotyrosine in control and pervanadate-treated A431 cells. The cells were labeled with rabbit polyclonal anti-Phosphotyrosine (PP2221) and mouse monoclonal anti-Phosphotyrosine (PM3751), then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.



Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

PM3801 Fester, L. et al. (2015) JSBMB S0960-0760(15)30109. WB: Hippocampal neurons
PM3801 Osma-Garcia, I.C. et al. (2015) Eur J Immunol. doi: 10.1002 WB: mouse macrophages
PP2221 Hamada-Kawaguchi, N. et al. (2015) PLoS One. 10(3):e0121484 ICC: fly ovary
PP2551 Kommaddi, R.P. et al. (2015) J Biol Chem. 3;290(14):8888 WB: COS-7 cells
PP2551 Tsai, C.F. et al. (2015) J Agric Food Chem. 9;63(48):10388 WB: Huh7 cells
PM3801 Morinaga, J. et al. (2013) AJP Renal Phys. 305(2)F173. WB: Normal Rat Kidney Fibroblast
PP2221 Sroyraya, M. et al. (2013) Microsc Res Tech. 76(1):103. WB: Swimming Crab
PP2551 Kim, S. et al. (2013) Diabetes. 62(2):471. WB, IP: mouse adipocytes
PP2551 Sroyraya, M. et al. (2013) Microsc Res Tech. 76(1):103. WB: Swimming Crab
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
PP2551 Hummel, S. et al. (2012) Appl Environ Microbiol. 78(4):1140. WB: human colonic adenocarcinoma cells
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
PP2551 Laluk, K. et al. (2011) Plant Cell 23(8):2831. WB: Arabidopsis BIK1 and Myelin basis protein
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
Anti-Phosphoserine/threonine Mouse pAb50 μlWB, E, IP, ICCHu, Rt, Ms
Anti-Phosphoserine/threonine Rabbit pAb50 μlWB, E, IP, ICCHu, Rt, Ms
Anti-Phosphotyrosine Mouse mAb50 μlWB, E, IP, ICCHu, Rt, Ms, Ck
Anti-Phosphotyrosine Rabbit pAb50 μlWB, E, IP, ICCHu, Rt, Ms, Ck
Anti-Mouse Ig:HRP Donkey pAb 100 μlWB, EMs
Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb 100 μlWB, E, ICC, IHCRb
KIT SUMMARY

The phospho-tyrosine, -serine, and -threonine antibody sampler kit can be used to detect changes in phosphorylation at tyrosine, serine, and threonine residues within proteins. The kit also includes secondary reagents for detection of these antibodies.