PTP1B is a nonreceptor type protein tyrosine phosphatase that has essential roles in insulin and leptin signaling, as well as important functions in growth factor and integrin signaling. The structure of PTP1B includes a conserved phosphatase domain, C-terminal hydrophobic residues for targeting to the cytoplasmic face of the endoplasmic reticulum, and proline-rich regions characteristic of SH3 domain binding motifs. PTP1B can interact with N-Cadherin and dephosphorylate β-catenin associated with cadherin complexes. PTP1B also interacts with Insulin and EGF receptors, and undergoes phosphorylation after receptor stimulation. Tyrosine phosphorylation at Tyr-66, Tyr-152, and Tyr-153 occurs after insulin receptor activation, and tyrosine phosphorylation of Tyr-152 may be required for interactions with N-Cadherin. In addition, Akt can phosphorylate Ser-50 and this phosphorylation can reduce PTP1B activity.
Lingamanaidu, V. et al. (2001) Molecular Endocrinology 15(10):1768.
Rhee, J. et al. (2001) J. Biol. Chem. 276(9):6640.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
PP2351 Obanda, D.N. et al. (2012) Diabetes 61(3):597. WB: L6 myoblasts
PP2411 Obanda, D.N. et al. (2012) Diabetes 61(3):597. WB: L6 myoblasts
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
This kit contains:
|CATALOG#||DESCRIPTION||SIZE||APPLICATIONS||SPECIES REACTIVITY||MW (kDa)|
|PTP1B (C-terminal region) Mouse mAb||50 μl||WB, E, ICC||Hu||50|
|PTP1B (a.a. 146-157) Rabbit pAb||50 μl||WB, E||Hu, Rt, Ms||50|
|PTP1B (Ser-50), phospho-specific Rabbit pAb||50 μl||WB, E||Hu, Rt, Ms||50|
|PTP1B (Tyr-152), phospho-specific Rabbit pAb||50 μl||WB, E||Hu, Rt, Ms||50|
|Anti-Mouse Ig:HRP Donkey pAb||100 μl||WB, E||Ms|
|Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb||100 μl||WB, E, ICC, IHC||Rb|
The PTP1B phospho-regulation antibody sampler kit can be used to detect phosphorylation of PTP1B at Ser-50 and Tyr-152. The kit also includes an antibody to examine total PTP1B expression levels and secondary reagents for rabbit polyclonal and mouse monoclonal antibody detection.