Catalog # PK6290


PTP1B Phospho-Regulation

Antibody Sampler Kit

$395

PTP1B is a nonreceptor type protein tyrosine phosphatase that has essential roles in insulin and leptin signaling, as well as important functions in growth factor and integrin signaling. The structure of PTP1B includes a conserved phosphatase domain, C-terminal hydrophobic residues for targeting to the cytoplasmic face of the endoplasmic reticulum, and proline-rich regions characteristic of SH3 domain binding motifs. PTP1B can interact with N-Cadherin and dephosphorylate β-catenin associated with cadherin complexes. PTP1B also interacts with Insulin and EGF receptors, and undergoes phosphorylation after receptor stimulation. Tyrosine phosphorylation at Tyr-66, Tyr-152, and Tyr-153 occurs after insulin receptor activation, and tyrosine phosphorylation of Tyr-152 may be required for interactions with N-Cadherin. In addition, Akt can phosphorylate Ser-50 and this phosphorylation can reduce PTP1B activity.

References
Bandyopadhyay, D. et al. (1997) J. Biol. Chem. 272(3):1639.
Lingamanaidu, V. et al. (2001) Molecular Endocrinology 15(10):1768.
Rhee, J. et al. (2001) J. Biol. Chem. 276(9):6640.
Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
PP2351 Obanda, D.N. et al. (2012) Diabetes 61(3):597. WB: L6 myoblasts
PP2411 Obanda, D.N. et al. (2012) Diabetes 61(3):597. WB: L6 myoblasts
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot

Western blot image of mouse SYF cSrc-transformed cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with pervanadate (1 mM for 30 min.). The blots were probed with rabbit polyclonal anti-PTP1B (a.a. 146-157) (lanes 1 & 2) or anti-PTP1B (Tyr-152) (lanes 3 & 4).

Western blot image of human Jurkat cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with calyculin A (100 nM for 30 min.). The blots were probed with monoclonal anti-PTP1B (lanes 1 & 2) or polyclonal anti-PTP1B (Ser-50) (lanes 3 & 4).



This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
PTP1B (C-terminal region) Mouse mAb50 μlWB, E, ICCHu50
PTP1B (a.a. 146-157) Rabbit pAb50 μlWB, EHu, Rt, Ms50
PTP1B (Ser-50), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms50
PTP1B (Tyr-152), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms50
Anti-Mouse Ig:HRP Donkey pAb 100 μlWB, EMs
Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb 100 μlWB, E, ICC, IHCRb
KIT SUMMARY

The PTP1B phospho-regulation antibody sampler kit can be used to detect phosphorylation of PTP1B at Ser-50 and Tyr-152. The kit also includes an antibody to examine total PTP1B expression levels and secondary reagents for rabbit polyclonal and mouse monoclonal antibody detection.