Catalog # PK6250

p130 Cas Phospho-Regulation Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395

p130 Cas (Crk-associated substrate (CAS), breast cancer antiestrogen resistance 1 (BCAR1)) is a docking protein containing multiple protein-protein interaction domains. The N-terminal SH3 domain functions as a molecular switch regulating CAS tyrosine phosphorylation, as it interacts with tyrosine kinases and phosphatases. The C-terminal Src binding domain contains a proline-rich motif that mediates interaction with the SH3 domains of Src-family kinases (SFKs). Phosphorylation of this domain at Tyr-762 in rat (Tyr-668 in mouse) promotes this interaction. The p130 Cas central substrate domain is characterized by 15 tyrosines present in Tyr-X-X-Pro (YXXP) motifs, including Tyr-165, Tyr-249, and Tyr-410. When phosphorylated, most YXXP motifs are able to serve as docking sites for proteins with SH2 or PTB domains. In addition, phosphorylation of Tyr-751 (Tyr-653 in human) near the C-terminal caspase recognition site can attenuate caspase cleavage, while dephosphorylation occurs during apoptosis and may facilitate p130 Cas degradation.

References
Pellicena, P. & Miller, W.T. (2001) J Biol Chem. 276 (30):28190.
Kim, D.H. et al. (2003). Biochem Biophys Res Comm. 300:141.
Shin, N-Y. et al. (2004). J Biol Chem. 279(37):38331.
Shin, N-Y. et al. (2004) J Biol Chem. 279 (37):38331.

Western blot analysis of human endothelial cells serum starved overnight (lanes 1, 3, & 5) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4, & 6). The blot was probed with anti-p130 Cas (PM1441; lanes 1 & 2), anti-p130 Cas (Tyr-751) (PP1581; lanes 3 & 4), or anti-p130 Cas (Tyr-762) (PP1451; lanes 5 & 6).

Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4-6). The blot was probed with anti-p130 Cas (lanes 1 & 2) or anti-p130 Cas (Tyr-762) (lanes 3-6). The latter was used in the presence of no peptide (lane 4), phospho-p130 Cas (Tyr-762) peptide (lane 5) or phospho-p130 Cas (Tyr-751) peptide (lane 6).



Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
PM1441 Wilke, G.A. & Wardenburg, J.B. (2010) PNAS. 107(30):13473. WB: human A459

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
p130 Cas(C-terminal region) Mouse mAb50 μlWB, E, IP, IHCHu, Rt, Ms, Ck130
p130 Cas (Tyr-751), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms130
p130 Cas (Tyr-751), phospho-specific Rabbit pAb 50 μlWB, EHu, Rt, Ms68
Anti-Mouse Ig:HRPDonkey pAb100 μlWB, EMs
Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb100 μlWB, E, ICC, IHCRb
KIT SUMMARY

The p130 Cas phospho-regulation antibody sampler kit can be used to detect p130 Cas phosphorylation at Tyr-751 and Tyr-762. The kit also includes an antibody to examine total p130 Cas expression levels and secondary reagents for rabbit polyclonal and mouse monoclonal antibody detection.