Catalog # PK6140

p38 MAPK Phospho-Regulation Antibody Kit

Antibody Sampler Kit



p38 MAP kinase (MAPK), also called RK, CSBP, and SAPK2a, is the mammalian orthologue of the yeast HOG kinase. This family of kinases participates in signaling cascades that control cellular responses to cytokines and stress. Four isoforms of p38 MAPK (α,β,γ,δ) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides, UV light, and growth factors. MKK3 and SEK activate p38 MAPK by dual phosphorylation at Thr-180/Tyr-182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2, Max, and MEF2. T cells possess an alternative pathway for p38α activation where stimulation of the antigen receptor (TCR) induces phosphorylation of p38 on Tyr-323. This site is required for TCR-mediated phosphorylation of Thr-180 and catalytic activity. Thus, Tyr-323 may also have important roles in regulating p38 MAP kinase pathways.

Freshney, N. W. et al. (1994) Cell 78:1039.
Raingeaud, J. et al. (1995) J. Biol. Chem. 270:7420.
Salvador, J.M. et al. (2005) Nat Immunol. 6(4):390.
Jirmanova, L. et al. (2009) Blood 113(10):2229.

A) Western blot image of GST-recombinant p38 (K53M) mutant kinase untreated (lanes 1 & 3) or treated with Fyn kinase (lanes 2 & 4). B) Western blot analysis of p38 phosphorylation in mouse macrophages stimulated with 1 mM pervanadate for 30 min. (lanes 1 & 3) then the blot was treated with alkaline phosphatase (lanes 2 & 4). Both blots were probed with anti-p38α (a.a. 319-328) (lanes 1 & 2) or anti-p38α (Tyr-323) (lanes 3 & 4).

Immunocytochemical labeling of p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38α MAPK and rabbit polyclonal p38 MAPK (Tyr-323) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

PP3411 Alam, M.S. et al. (2015) Nat Medicine 21:1337. WB, IF: human T-cells, CD3 activated
PP3411 Lanna, A. et al. (2014) Nat Immunol. 15(10):965. WB: human T-cells, CD3 activated
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
PM1391 Chambers, M.A. et al. (2009) J Physiol. 587:3363. WB: mouse muscle, C2C12
PM1391 Li, W. et al. (2009) AJP Cell Phys. 297:C706. WB: mouse C2C12 cells

This kit contains:

p38aMAP Kinase (a.a. 319-328) Rabbit pAb50 μlWB, EHu, Rt, Ms, Ck38
p38aMAP Kinase (C-terminal) Mouse mAb50 μlWB, E, ICCHu, Rt, Ms38
p38aMAP Kinase (Thr-180/Tyr-182), phospho-specific Mouse mAb 50 μlWB, E, ICCHu, Rt, Ms38
p38aMAP Kinase (Tyr-323), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck38
Anti-Mouse Ig:HRP Donkey pAb 100 μlWB, EMs
Anti-Rabbit Ig Light ChainSpecific:HRP Mouse mAb 100 μlWB, E, ICC, IHCRb

The p38 phospho-regulation antibody sampler kit can be used to detect p38 phosphorylation at Thr-180/Tyr-182 and Tyr-323. The kit also includes an antibody to examine total p38 expression levels and secondary reagents for rabbit polyclonal and mouse monoclonal antibody detection.