Catalog # PK6070


Paxillin Phospho-Regulation

Antibody Sampler Kit

$395

Paxillin is a focal adhesion protein involved in cell adhesion and migration. Phosphorylation of serine and tyrosine sites in paxillin regulates its activity. Serine phosphorylation occurs in response to growth-factor and integrin activation. Both JNK1 and cdc2 kinases can phosphorylate Ser-178, and this phosphorylation may be important for cell migration, while ERK and p38MAPK kinases phosphorylate Ser-83, and this phosphorylation may be important for HGF-induced cell spreading and NGF-induced neurite extension. Tyrosine phosphorylation of paxillin also occurs in response to growth factor and integrin activation. The major sites of tyrosine phosphorylation include Tyr-31 and Tyr-118, and both of these sites may be involved in integrin-mediated cell adhesion.

References
Huang, C. et al. (2003) Nature 424:219-223.
Huang, C. et al. (2004) J Cell Biol. 164(4):593-602.
Huang, C. et al. (2004) Cell Cycle 3(1):4-6.
Ishibe, S. et al. (2004) Mol. Cell 16 :257-267.
Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

PM1071 Omori, K. et al. (2015) Bioch Biophys Res Com 458(4): 934. IF: mouse NIH3T3 fibroblasts
PM1071 Cheng, I. et al. (2014) J Biol Chem. 289(39):26989 IF: B16-F1 cells
PP1051 Wu, D. W. et al. (2014) Oncogene 33(35): 4385. WB: CCM3 cells
PP1341 Wu, D. W. et al. (2014) Oncogene 33(35): 4385. WB: CCM3 cells
PM1021 Lee, S. et al. (2013) Biochim Biophys Acta. 1830(8):4017. WB: rat C6 and human U373 gliomas
PM1071 Lee, S. et al. (2013) Biochim Biophys Acta. 1830(8):4017. WB: rat C6 and human U373 gliomas
PM1071 Liu, H. et al. (2013) BMC Cancer. 13:80. WB: human ovarian cancer cells
PP1051 Liu, H. et al. (2013) BMC Cancer. 13:80. WB: human ovarian cancer cells
PP1051 Wei, W. et al. (2013) Pancreatology 13(4):384. WB: pancreatic PANC1, Capan-2 and HT1080 cells
PM1021 Zou, W. et al. (2012) J Bone Miner Res. 27(12):2490. WB: mouse bone marrow macrophages
PP1341 Sen, A. et al. (2012) J Clin Invest. 122(7):2469. WB, ICC: C4C2, PC3, LNCaP
PP1051 Kwak, T.K. et al. (2010) J Biol Chem. 285(46):36021. WB: pancreatic INS-1 cells
PP1341 Kwak, T.K. et al. (2010) J Biol Chem. 285(46):36021. WB: pancreatic INS-1 cells
PP1341 Sen, A. et al. (2010) J Biol Chem. 285(37):28787. WB: human LNCAP cells

Western blot analysis of A431 cells (30 μg/lane) serum starved overnight (lane 1 & 3) or treated with pervanadate (1 mMl) for 30 min (lanes 2 & 4). The blot was probed with anti-Paxillin (PM1071) or anti-phospho-Paxillin (Tyr-31) (PM1021).

Immunocytochemical labeling of Ser-83 phosphorylated paxillin in rabbit spleen fibroblasts. The cells were labeled with mouse monoclonal Paxillin (left) and rabbit polyclonal Paxillin (Ser-83, right) antibodies, then detected using appropriate secondary antibodies conjugated to Cy3.



This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
Paxillin Mouse mAb50 μlWB, E, IP, ICCHu, Rt, Ms, Ck68
Paxillin (C-terminal) Rabbit pAb50 μlWB, EHu, Rt, Ms68
Paxillin (Tyr-31), phospho-specific Mouse mAb50 μlWB, EHu, Rt, Ms68
Paxillin (Ser-83), phospho-specific Rabbit pAb50 μlWB, E, ICCRt, Ms68
Paxillin (Ser-178), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms68
KIT SUMMARY

The paxillin phospho-regulation antibody sampler kit can be used to examine phosphorylation of paxillin at Tyr-31, Ser-83, and Ser-178. The kit also includes monoclonal and polyclonal antibodies to monitor total paxillin expression levels