Catalog # MK7730
Myosin Light Chain Phospho-Regulation Staining Kit
Immunocytochemistry Kit
| ELISA, WB, ICC, IHC |
Species Reactivity
| Hu, Rt, Ms, Ck, Cw, Rb |
 |
KIT SUMMARY The Myosin Regulatory Light Chain phospho-regulation kit can be used for immunocytochemical co-localization of MLC phosphorylation at Ser-1 and Ser-19 compared to the total expression of MLC. The kit includes rabbit polyclonal antibodies along with Goat-anti-rabbit conjugated to DyLight® 594 . |
Immunocytochemical labeling of phosphorylated MLC in paraformaldehyde fixed A7r5 cells. The cells were dual-labeled with anti-MLC (MM3441; middle) and anti-MLC (MP4201; top left), anti-MLC (Ser-19) (MP4221; middle left) and anti-MLC (Ser-1) (MP3461; bottom left). Goat anti-Mouse DyLight® 488 and Goat anti-Rabbit DyLight® 594 were used for detection of primary antibodies. The overlay of staining patterns are shown to the right.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
MP4221 Ho, H. et al. (2013) Pigment Cell Melanoma Res 26(2):218. WB: human A375 melanomaMP3461 Aguilar, H.N. et al. (2011) PLoS ONE 6(6):e20903. WB: human uterine myocytes
MP3461 Beach, J.R. et al. (2011) BMC Cell Biol. 12:52. ICC: PHK and HeLa cells
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | ICC DILUTION |
| Myosin Light Chain (a.a. 11-22) Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, Ck | 1:200 | |
| Myosin Light Chain (Ser-1), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, Cw | 1:100 | |
| Myosin Light Chain (Ser-19), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, Ck | 1:100 | |
| Anti-Rabbit Ig:DyLight® 594 Goat pAb | 100 μl | ICC, IHC | Rb | 1:200 |
