Catalog # MK7730

Myosin Light Chain Phospho-Regulation Staining Kit

Immunocytochemistry Kit

Application / Dilution
ELISA, WB, ICC, IHC

Species Reactivity

Hu, Rt, Ms, Ck, Cw, Rb

$455


DATASHEET  

KIT SUMMARY

The Myosin Regulatory Light Chain phospho-regulation kit can be used for immunocytochemical co-localization of MLC phosphorylation at Ser-1 and Ser-19 compared to the total expression of MLC. The kit includes rabbit polyclonal antibodies along with Goat-anti-rabbit conjugated to DyLight® 594 .

Immunocytochemical labeling of phosphorylated MLC in paraformaldehyde fixed A7r5 cells. The cells were dual-labeled with anti-MLC (MM3441; middle) and anti-MLC (MP4201; top left), anti-MLC (Ser-19) (MP4221; middle left) and anti-MLC (Ser-1) (MP3461; bottom left). Goat anti-Mouse DyLight® 488 and Goat anti-Rabbit DyLight® 594 were used for detection of primary antibodies. The overlay of staining patterns are shown to the right.



Rabbit polyclonals and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

MP4221 Ho, H. et al. (2013) Pigment Cell Melanoma Res 26(2):218. WB: human A375 melanoma
MP3461 Aguilar, H.N. et al. (2011) PLoS ONE 6(6):e20903. WB: human uterine myocytes
MP3461 Beach, J.R. et al. (2011) BMC Cell Biol. 12:52. ICC: PHK and HeLa cells

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYICC DILUTION
Myosin Light Chain (a.a. 11-22) Rabbit pAb 50 μlWB, E, ICCHu, Rt, Ms, Ck1:200
Myosin Light Chain (Ser-1), phospho-specific Rabbit pAb 50 μlWB, E, ICCHu, Rt, Ms, Cw1:100
Myosin Light Chain (Ser-19), phospho-specific Rabbit pAb 50 μlWB, E, ICCHu, Rt, Ms, Ck1:100
Anti-Rabbit Ig:DyLight® 594 Goat pAb100 μlICC, IHCRb1:200