Catalog # MK7640

Microtubule Staining Kit

Immunocytochemistry Kit

Application / Dilution
ELISA, WB, ICC, IHC

Species Reactivity

Hu, Rt, Ms, Rb

$355


DATASHEET  

KIT SUMMARY

The microtubule lableing kit can be used for immunocytochemical labeling of tubulin. The kit includes monoclonal antibodies to α- and βI-tubulin, and a rabbit polyclonal antibody to βIII-tubulin. The kit also includes goat-anti-rabbit conjugated to DyLight® 594 and goat anti-mouse conjugated to DyLight® 488 for dual labeling experiments.

Immunocytochemical labeling of Tubulin isoforms. In rat A7r5 cells, microtubules were labeled using anti-βI-Tubulin (TM1541) (left) and anti-α-tubulin (TM4111) (right). In NGF-differentiated rat PC12 cells, microtubules were labeled using anti-βIII-Tubulin (TP1691) (middle). The antibodies were detected using the appropriate secondary antibody conjugated to DyLight® 488 or 594.



Mouse monoclonal, rabbit polyclonal, and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

TP1691 Sitaras, N. et al. (2015) Am J Pathol. 185(2):581 IF: mouse retinal ganglion cells
TM1541 Wang, Q. et al. (2014) J Virol. 88(9):4853 WB: HeLa
TM4111 Gehrig, S.M. et al. (2012) Nature. 484(7394):394. WB: mouse skeletal muscle
TP1691 Saphieha, P. et al. (2012) Nutr Diabetes. 2:36. ICC: mouse retina cells
TM1541 Meacham, CE. et al. (2011) Nature Genetics. 41:1133. WB: mouse lymphoma
TM1541 Meshki, J. et al. (2011) PLoS ONE. 6(9):e25332. WB: U3&3MG
TM1541 Campbell, S. et al. (2010) J Biol Chem. 285:4695. WB: human HeLa cells

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYICC DILUTION
α-Tubulin (C-terminus) Mouse mAb 50 μlWB, E, IP, ICC, IHCHu, Rt, Ms1:200
β-TubulinMouse mAb 50 μlWB, E, ICCHu, Rt, Ms1:200
βIII-Tubulin (C-terminus) Rabbit pAb50 μlWB, E, ICC, IHCHu, Rt, Ms1:200
Anti-Mouse Ig:DyLight® 488 Goat pAb100 μlICC, IHCMs1:200
Anti-Rabbit Ig:DyLight® 594 Goat pAb100 μlICC, IHCRb1:200