Brain (cerebrum and cerebellum), gastrocnemius muscle, whole heart, kidney, liver, and spleen were dissected from adult mouse (Balb/c). The tissues were rinsed in Hank’s buffered saline solution and lysed in boiling 1% SDS, 1.0 mM sodium ortho-vanadate, 10 mM Tris (pH 7.4) buffer. Each lysate was sonicated then diluted to the final concentration in electrophoresis sample buffer.
Dennis-Sykes, C.A. et al. (1985) J Biol Stand. 13(4):309.
Burgoyne, R.D.et al. (1988) EMBO J. 7(8):2311.
MacPhee, D.J. (2010) J Pharmacol Toxicol Methods. 61(2):171.
Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.
Western blot analysis of adult mouse tissue lysates (20 μg/lane): brain (lane 1), spleen (lane 2), liver (lane 3), kidney (lane 4), muscle (lane 5), and heart (lane 6). The blots were probed with rabbit polyclonal anti-Actin (AP1651).
Western blot analysis of adult mouse tissue lysates (20 μg/lane): brain (lane 1), spleen (lane 2), liver (lane 3), kidney (lane 4), muscle (lane 5), and heart (lane 6). The blots were probed with mouse monoclonal anti-Paxillin (PM1071).
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot
mobilities of known proteins with similar MW.
This kit contains:
|Mouse Brain||Lysate||100 μl||WB|
|Mouse Heart||Lysate||100 μl||WB|
|Mouse Kidney||Lysate||100 μl||WB|
|Mouse Liver||Lysate||100 μl||WB|
|Mouse Muscle||Lysate||100 μl||WB|
|Mouse Spleen||Lysate||100 μl||WB|
The mouse tissue lysate sampler kit contains Western blot lysates from several adult mouse (Balb/c) tissues, including brain, muscle, heart, kidney, liver, and spleen. This kit can be used to examine patterns of mouse protein expression.