Catalog # MK6900

Mouse Tissue Kit

Lysate Kit

DATASHEET  




$295
Brain (cerebrum and cerebellum), gastrocnemius muscle, whole heart, kidney, liver, and spleen were dissected from adult mouse (Balb/c). The tissues were rinsed in Hank’s buffered saline solution and lysed in boiling 1% SDS, 1.0 mM sodium ortho-vanadate, 10 mM Tris (pH 7.4) buffer. Each lysate was sonicated then diluted to the final concentration in electrophoresis sample buffer.
References
Dennis-Sykes, C.A. et al. (1985) J Biol Stand. 13(4):309.
Burgoyne, R.D.et al. (1988) EMBO J. 7(8):2311.
MacPhee, D.J. (2010) J Pharmacol Toxicol Methods. 61(2):171.

Western blot analysis of adult mouse tissue lysates (20 μg/lane): brain (lane 1), spleen (lane 2), liver (lane 3), kidney (lane 4), muscle (lane 5), and heart (lane 6). The blots were probed with rabbit polyclonal anti-Actin (AP1651).

Western blot analysis of adult mouse tissue lysates (20 μg/lane): brain (lane 1), spleen (lane 2), liver (lane 3), kidney (lane 4), muscle (lane 5), and heart (lane 6). The blots were probed with mouse monoclonal anti-Paxillin (PM1071).



Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

CATALOG#DESCRIPTIONPRODUCT TYPESIZEAPPLICATIONS
Mouse BrainLysate100 μlWB
Mouse HeartLysate100 μlWB
Mouse KidneyLysate100 μlWB
Mouse LiverLysate100 μlWB
Mouse MuscleLysate100 μlWB
Mouse SpleenLysate100 μlWB
KIT SUMMARY

The mouse tissue lysate sampler kit contains Western blot lysates from several adult mouse (Balb/c) tissues, including brain, muscle, heart, kidney, liver, and spleen. This kit can be used to examine patterns of mouse protein expression.