Catalog # MK6660


MeCP2 Phospho-Regulation

Antibody Sampler Kit

$395

Methyl-CpG Binding Protein 2 (MeCP2) was identified based on its affinity for methylated cytosines within DNA. As a chromatin-associated multifunctional protein, MeCP2 has been implicated in regulation of transcription and chromatin structure. Mutations of MeCP2 cause Rett syndrome, which results from neuronal dysfunction and impairment in cognitive and motor functions. Regulation of MeCP2 activity may involve phosphorylation at multiple sites. Ser-421 in MeCP2 is phosphorylated in response to neuronal activity, calcium influx, and is dependent on Cam-KII. Alanine mutation of Ser-421 leads to defects in synapse development and activity. Ser-80 in MeCP2 is phosphorylated in HeLa nuclear extracts and neurons. Alanine mutation of Ser-80 attenuates MeCP2 chromatin association and leads to locomotor deficits in transgenic knock-in mice. Thus, phosphorylation of MeCP2 may be important for altering its function during neuronal activity.

References
Cohen, S. et al. (2011) Neuron 72:72.
Tao, J. et al. (2009) PNAS 106(12):4882.
Lewis, J.D. et al. (1992). Cell 69:905.
Rabbit polyclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The peptides are supplied in PBS and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

MP4611 Wang, A. et al. (2014) PloS one 9(4): e94394. doi: 10.1371 WB: rat hippocampal CP1

Western blot of adult mouse brain tissue lysate. The blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonals anti-MeCP2 (Ser-80) (lanes 1 & 2) or anti-MeCP2 (C-terminus) (lanes 3 & 4).

Immunocytochemical labeling of MeCP2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with anti-MeCP2 (Ser-421) (MP4611) in the absence (left) or presence (right) of blocking peptide (MX4615). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.



This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
MeCP2 (C-terminus) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms75
MeCP2 (Ser-80), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms75
MeCP2 (Ser-421), phospho-specific Rabbit pAb 50 μlWB, E, ICCHu, Rt, Ms75
MeCP2 (C-terminus) Peptide50 μg AB, E
phospho-MeCP2 (Ser-80) Peptide50 μgAB, E
phospho-MeCP2 (Ser-421) Peptide 50 μgAB, E
KIT SUMMARY

The MeCP2 phospho-regulation kit can be used to detect phosphorylation of Ser-80 and Ser-421 in MeCP2. The kit includes an anti-MeCP2 (C-terminus) antibody for comparison total MeCP2 expression relative to site-specific phosphorylation level. In addition, the kit includes blocking peptides for examining the specificity of the antibodies in western blot and immunocytochemistry.