Catalog # MK6050

MAP Kinase Activation Antibody Kit

Antibody Sampler Kit

Application / Dilution

Species Reactivity

Hu, Rt, Ms




The MAP kinase activation antibody sampler kit can be used to examine the activation of three major MAPKs: p38, ERK, and JNK. The kit also includes antibodies to monitor total expression levels of p38, ERK1/2, and JNK1.

Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) then treated with pervanadate (1 mM) for 30 min. (lanes 2 & 4). The blot was probed with anti-p38α (lanes 1 & 2) or anti-p38 (T180/Y182) (lanes 3 & 4).

Western blot analysis of human A431 cells untreated (lanes 1 & 4), treated with 100 nM calyculin A for 30 min. (lanes 2 & 5), or 100 ng/ml EGF for 60 min. (lanes 3 & 6). The blots were probed with anti-ERK1 (lanes 1, 2, & 3) or anti-ERK1/2 (Thr-202/Tyr-204) (lanes 4, 5, & 6).

Mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

EM2331 Kyjacova, L. et al. (2015) Cell Death Differ. 22(6):898. WB: human DU145
EM2061 Park, K. et al. (2013) Mol Cell Biol. 33(4):752. WB: human keratinocytes
PM1391 Chambers, M.A. et al. (2009) J Physiol. 587:3363. WB: mouse muscle, C2C12
PM1391 Li, W. et al. (2009) AJP Cell Phys. 297:C706. WB: mouse C2C12 cells

This kit contains:

ERK1 (C-terminal region) Mouse mAb50 μlWB, E, ICC, IHCHu, Rt, Ms44
ERK1 (Thr-202/Tyr-204)[conserved], phospho-specific Mouse mAb 50 μlWB, E, ICCHu, Rt, Ms44
JNK1 (C-terminal region) Mouse mAb 50 μlWB, E, ICC, IHCHu, Rt, Ms42
JNK (Thr-183/Tyr-185), phospho-specific Mouse mAb50 μlWB, E, ICCHu, Rt, Ms42
p38 (C-terminal) Mouse mAb50 μlWB, E, ICCHu, Rt, Ms38
p38 MAP Kinase (Thr-180/Tyr-182)), phospho-specific Mouse mAb50 μlWB, E, ICCHu, Rt, Ms28