Catalog # LK6380


LIMK Phospho-Regulation

Antibody Sampler Kit

$395

LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains. LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK. PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr-508 or Thr-505 residues in the activation loop, increasing LIMK activity. In addition, VEGF-induced stress fiber formation has been linked to p38-mediated activation of LIMK through MK-2 phosphorylation of Ser-323. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser-3 residue of cofilin. In addition, LIMKs may have a function in the nucleus. It has been shown that the nuclear localization of LIMKs can mediate suppression of Rac/Cdc42-mediated cyclin D1 expression. This effect of LIMKs was independent of cofilin phosphorylation and the regulation of actin dynamics.

References
Okano, I. et al. (1995) J. Biol. Chem. 270:31321.
Edwards, D. C. et al. (1999) Nat. Cell Biol. 1:253.
Kobayashi, M. et al. (2006) EMBOJ 25:713.
Rabbit polyclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The peptides are supplied in PBS and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

LP1891 Marshall, T.W. et al. (2009) J Cell Sci. 122:3061. WB: rat MTLn3 cells

Western blot image of human A431 cells untreated (lanes 1 & 3) or treated (lanes 2 & 4)with calyculin A (100 nM for 30 min) . The blots were probed with anti-LIMK1 (C-terminus) (lanes 1 & 2) or anti-LIMK1 (Ser-323) (lanes 3 & 4).

Western blot of activated mouse recombinant LIMK1 untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-LIMK1 (C-terminus) (lanes 1 & 2; LP1831) or anti-LIMK1 (Thr-508) (lanes 3 & 4; LP1891).



This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
LIMK1 (C-terminus) Rabbit pAb50 μlWB, EHu, Rt, Ms72
LIMK1 (Ser-323)[LIMK2 (Ser-314)], phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms72
LIMK1 (Thr-508)[LIMK2 (Thr-505)], phospho-specific Rabbit pAb 50 μlWB, EHu, Rt, Ms72
LIMK1 (C-terminus) Peptide50 μg AB, E
phospho-LIMK1 (Ser-323) Peptide50 μgAB, E
phospho-LIMK1 (Thr-508) Peptide 50 μgAB, E
KIT SUMMARY

The LIMK phospho-regulation antibody sampler kit can be used to monitor the level of phosphorylated LIMK1 relative to total LIMK1 expression. In addition, the kit provides peptides for antibody blocking experiments