Catalog # LK6380

LIMK Phospho-Regulation Antibody Kit

Antibody Sampler Kit

Application / Dilution
ELISA, WB, AB

Species Reactivity

Hu, Rt, Ms

$395


DATASHEET  

KIT SUMMARY

The LIMK phospho-regulation antibody sampler kit can be used to monitor the level of phosphorylated LIMK1 relative to total LIMK1 expression. In addition, the kit provides peptides for antibody blocking experiments

Western blot image of human A431 cells untreated (lanes 1 & 3) or treated (lanes 2 & 4)with calyculin A (100 nM for 30 min) . The blots were probed with anti-LIMK1 (C-terminus) (lanes 1 & 2) or anti-LIMK1 (Ser-323) (lanes 3 & 4).

Western blot of activated mouse recombinant LIMK1 untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-LIMK1 (C-terminus) (lanes 1 & 2; LP1831) or anti-LIMK1 (Thr-508) (lanes 3 & 4; LP1891).



Rabbit polyclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The peptides are supplied in PBS and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

LP1891 Marshall, T.W. et al. (2009) J Cell Sci. 122:3061. WB: rat MTLn3 cells

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
LIMK1 (C-terminus) Rabbit pAb50 μlWB, EHu, Rt, Ms72
LIMK1 (Ser-323)[LIMK2 (Ser-314)], phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms72
LIMK1 (Thr-508)[LIMK2 (Thr-505)], phospho-specific Rabbit pAb 50 μlWB, EHu, Rt, Ms72
LIMK1 (C-terminus) Peptide50 μg AB, E
phospho-LIMK1 (Ser-323) Peptide50 μgAB, E
phospho-LIMK1 (Thr-508) Peptide 50 μgAB, E