Catalog # JK7630

JNK Phospho-Regulation Staining Kit

Immunocytochemistry Kit

DATASHEET  




$355

The stress-activated protein kinases (SAPK) or Jun-amino-terminal kinases (JNK) are potently activated by stressors such as UV and gamma radiation. Similar to other MAP Kinases, the core signaling unit is composed of a MAPKKK, usually MEKK1-4 or a mixed lineage kinase (MLK), which phosphorylate and activate MKK4-7, leading to dual phosphorylation and activation of JNK kinases. Rho-GTPases (Rac1 and cdc42) can stimulate MEKKs and MLKs, while MKKs can be activated by a GTPase-independent pathway that involves the germinal center kinase family. There are three JNK genes (JNK1,2,3) with further diversification resulting from alternative splicing. Active JNK dimers can translocate to the nucleus to regulate transcription through phosphorylation of c-Jun, ATF-2, and other transcription factors.

References
Whitmarsh, A.J. & Davis, R.J. (1998) Trends Biochem. Sci. 23:481.
Ichijo, H. (1999) Oncogene 18:6087.
Kyriakis, J.M. (1999) J. Biol. Chem. 274:5259.
Davis, R.J. (1999) Biochem. Soc. Symp. 64:1.

Immunocytochemical labeling of JNK in control (left) or calyculin A-treated A431 cells (right). The cells were labeled with mouse monoclonal JNK (C-terminal region) (top) or mouse monoclonal JNK (Thr-183/Tyr-185) (bottom). The antibodies were detected using goat anti-mouse DyLight® 594 and DyLight® 488, respectively.



Mouse monoclonal and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYICC DILUTION
JNK1 (C-terminal region) Mouse mAb50 μlWB, E, ICC, IHCHu, Rt, Ms1:100
JNK (Thr-183)/ (Tyr-185), phospho-specific Mouse mAb50 μlWB, E, ICCHu, Rt, Ms 1:100
Anti-Mouse Ig:DyLight® 488 Goat pAb100 μlICC, IHCMs1:200
Anti-Mouse Ig:DyLight® 594 Goat pAb100 μlICC, IHCMs1:200
KIT SUMMARY

The JNK1 phospho-regulation kit may be used to examine the pattern of phosphorylation of JNK1 at the dual site (Thr-183/Tyr-185) relative to the total JNK1 pattern. The kit includes monoclonal antibodies to JNK1 (C-terminal region) and JNK1 (Thr-183/Tyr-185), as well as Goat anti-mouse secondary reagents conjugated to DyLight® 488 or DyLight® 594 for detection using green (493Ex/518Em) or red (593Ex/618Em) filter sets.