Catalog # IK6270

Integrin β4 Phospho-Regulation Antibody Kit

Antibody Sampler Kit



Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin α6β4 receptors are found in basement membrane along with laminin-5. These receptors are expressed in epithelial, schwann, endothelial and some immune cells. The cytoplasmic domain of the integrin-β4 subunit recruits the adaptor protein Shc and is required for assembly of hemidesmosomes. Tyrosine phosphorylation of multiple sites within the cytoplasmic domain regulates these cellular events. In particular, tyrosine 1526 interacts with the phosphotyrosine binding domain of Shc and is required for Shc activation. In addition, tyrosine 1494 is required for integrin-mediated IRS-2 phosphorylation and activation of PI3-kinase. More importantly, this site is critical for integrin α6β4 increases in carcinoma invasion.


Product References:

Dans, M. et al. (2001). J Biol Chem. 276(2):1494-1502.
Shaw, L.M. (2001). Mol Cell Biol. 21(15):5082-5093.
Wang, L. et al. (2012) J Cell Physiol. 227(2):474.

Western blot analysis of A431 cells serum starved overnight (lanes 1, 3, & 5) and treated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal anti-integrin β4 (Tyr-1526) (lanes 1 & 2) and anti-integrin β4 (Tyr-1494) (lanes 3 & 4) or with mouse monoclonal anti-integrin β4 (lanes 5 & 6).

Immunocytochemical labeling of integrin β4 in control (Top) and pervanadate-treated A431 cells (Bottom). The cells were labeled with mouse monoclonal anti-integrin β4 (Cytoplasmic region) (left) or rabbit polyclonals anti-integrin β4 (Tyr-1494) (middle) or anti-integrin β4 (Tyr-1526) (right), then the antibodies were detected using appropriate secondary antibodies conjugated to DyLight® 594.

Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

IP1281 Coleman, D.T. et al. (2015) PLoS One. 10(5):e0125399 WB: HCC-1806, MCF-10A
IP1281 Soung, Y.H. et al. (2013) BMC Cell Biol. 1:14. WB: MDA-MB-231, MDA-MB-435
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
IP1281 Wang, L. et al. (2012) J Cell Physiol. 227(2):474.
IP1281 Soung, Y. & Chung, J. (2011) Mol Cancer Ther. 10(5):883. WB: human A431, MDA-MB-231
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
IP1281 Yang, X. et al. (2010) Mol Cell Biol. 30(22):5306. WB: Y1494F mutants
IP1281 Kim, H.I. et al. (2008) Cancer Prev Res. 1(5):385. WB: human b4 transfectants

This kit contains:

Integrin β4(Cytoplasmic region) Mouse mAb50 μlWB, E, ICCHu200
Integrin β4 (Tyr-1494), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms200
Integrin β4 (Tyr-1526), phospho-specific Rabbit pAb 50 μlWB, E, ICCHu, Rt, Ms200
Anti-Mouse Ig:HRPDonkey pAb100 μlWB, EMs
Anti-Rabbit Ig Light ChainSpecific:HRP Mouse mAb100 μlWB, E, ICC, IHCRb