Girdin, a member of the CCDC88 (Hook related protein) family, is an actin binding protein involved with cell migration and maintaining cytoskeletal organization. Girdin has conserved domains at the N- and C-terminus that bind microtubules and actin, respectively. It enhances PI3-kinase dependent phosphorylation of proteins, most notably Akt. This same activity can contribute to tumor proliferation, invasion, and metastasis in breast, ovarian, prostate, and pancreatic tissues. Girdin is phosphorylated at three separate locations: Ser-1416, Ser-1674, and Tyr-1764. Ser-1416 is the primary Akt phosphorylation site, while Cyclin-dependent kinases interact with Girdin and phosphorylate Ser-1674. Multiple receptor tyrosine kinases can bind girdin and phosphorylate Tyr-1764.
References
Bhandari, D. et al. (2015)Proc Natl Acad Sci. 112(35):E4874
Lin, C. et al. (2014) Mol Biol Cell. 25(22):3654
Lin, C. (2011) Sci Signal. 4(192): ra64
Western blot image of human A431 cell lysates treated with pervanadate (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Tyr-1764), phospho-specific (lanes 3 & 4).
Western blot image of human A431 cell lysates treated with Calyculin A (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Ser-1674), phospho-specific (lanes 3 & 4).
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblastsMS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| Girdin (C-terminus) Mouse mAb | 50 μl | WB, IP, ICC, IHC | Hu | 250 | |
| Girdin (Ser-1416) Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 250 | |
| Girdin (Ser-1674) Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 250 | |
| Girdin (Tyr-1764), phospho-specific Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 250 | |
| Anti-Mouse Ig:HRP Donkey pAb | 100 μl | WB, E | Ms | ||
| Anti-Rabbit Ig Light ChainSpecific:HRP Mouse mAb | 100 μl | WB, E, ICC, IHC | Rb |
KIT SUMMARY
The Girdin phospho-regulation antibody sampler kit can be used to detect phosphorylation of Ser-1416, Ser-1674 and Tyr-1764 relative to total Girdin expression levels. The kit includes rabbit polyclonal, phospho-specific antibodies to Ser-1416, Ser-1674, and Tyr-1764, as well as a mouse monoclonal antibody to detect total Girdin epxression. The kit also includes secondary reagents for detection of these antibodies in Western blot, ELISA, or immunocytochemistry.
