Catalog # EK7620

ERK1/2 Phospho-Regulation Staining Kit

Immunocytochemistry Kit

Application / Dilution

Species Reactivity

Hu, Rt, Ms




The ERK1/2 phospho-regulation kit may be used to examine the pattern of phosphorylation of ERK1 at the dual site (Thr-202/Tyr-204) relative to the total ERK1 pattern. The kit includes monoclonal antibodies to ERK1 (C-terminal region) and ERK1/2 (Thr-202/Tyr-204), as well as Goat anti-mouse secondary reagents conjugated to DyLight® 488 or DyLight® 594 for detection using green (493Ex/518Em) or red (593Ex/618Em) filter sets.

Immunocytochemical labeling of phosphorylated ERK1 in paraformaldehyde-fixed and NP-40-permeabilized rat A7r5 cells treated with calyculin A. The fixed cells were labeled with mouse monoclonal antibodies to anti-ERK1 (EM2331) and anti-ERK1/2 (Thr-202/Tyr-204) (EM2061). The antibodies were detected using Goat anti-Mouse secondary antibodies conjugated to DyLight® 488 (left) and DyLight® 594 (right).

Mouse monoclonal and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

EM2331 Kyjacova, L. et al. (2015) Cell Death Differ. 22(6):898. WB: human DU145
EM2061 Park, K. et al. (2013) Mol Cell Biol. 33(4):752. WB: human keratinocytes

This kit contains:

ERK1 (C-terminal region) Mouse mAb50 μlWB, E, ICC, IHCHu, Rt, Ms1:100
ERK1 (Thr-220)/ (Tyr-204)[conserved], phospho-specific Mouse mAb50 μlWB, E, ICCHu, Rt, Ms 1:100
Anti-Mouse Ig:DyLight® 488 Goat pAb100 μlICC, IHCMs1:200
Anti-Mouse Ig:DyLight® 594 Goat pAb100 μlICC, IHCMs1:200