Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The ERK1/2 (p44/42) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK), a MAP kinase kinase (MAPKK), and a MAP kinase (MAPK). Multiple ERK1/2 MAPKKKs have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate ERK1 and ERK2 through phosphorylation of activation loop residues Thr-202/Tyr-204 and Thr-185/Tyr-187, respectively. ERK1/2 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases. Several downstream targets of ERK1/2 have been identified, including p90RSK and the transcription factor Elk-1.
Murphy, L.O. & Blenis, J. (2006) Trends Biochem Sci 31:268.
Owens, D.M. & Keyse, S.M. (2007) Oncogene 26:3203.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:EM2331 Kyjacova, L. et al. (2015) Cell Death Differ. 22(6):898. WB: human DU145
EM2061 Park, K. et al. (2013) Mol Cell Biol. 33(4):752. WB: human keratinocytes
This kit contains:
|CATALOG#||DESCRIPTION||SIZE||APPLICATIONS||SPECIES REACTIVITY||ICC DILUTION|
|ERK1 (C-terminal region) Mouse mAb||50 μl||WB, E, ICC, IHC||Hu, Rt, Ms||1:100|
|ERK1 (Thr-220)/ (Tyr-204)[conserved], phospho-specific Mouse mAb||50 μl||WB, E, ICC||Hu, Rt, Ms||1:100|
|Anti-Mouse Ig:DyLight® 488 Goat pAb||100 μl||ICC, IHC||Ms||1:200|
|Anti-Mouse Ig:DyLight® 594 Goat pAb||100 μl||ICC, IHC||Ms||1:200|
The ERK1/2 phospho-regulation kit may be used to examine the pattern of phosphorylation of ERK1 at the dual site (Thr-202/Tyr-204) relative to the total ERK1 pattern. The kit includes monoclonal antibodies to ERK1 (C-terminal region) and ERK1/2 (Thr-202/Tyr-204), as well as Goat anti-mouse secondary reagents conjugated to DyLight® 488 or DyLight® 594 for detection using green (493Ex/518Em) or red (593Ex/618Em) filter sets.