Catalog # EK6960

EB3 Phospho-Regulation Antibody Kit

Antibody Sampler Kit

Application / Dilution
ELISA, WB, ICC

Species Reactivity

Hu, Rt, Ms

$395


DATASHEET  

KIT SUMMARY

The end-binding 3 (EB3) phospho-regulation antibody sampler kit can be used to detect the phosphorylation of Ser-162 and Ser-176 in EB3. The kit includes phospho-specific antibodies, as well as rabbit polyclonal and rat monoclonal antibodies for detecting total EB3 expression.

Western blot analysis of adult mouse brain untreated (lanes 1, 3, 5, & 7) or treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with rat monoclonal anti-EB3 (EM5091) (lanes 1 & 2), and rabbit polyclonals anti-EB3 (Ser-162) (lanes 3 & 4), anti-EB3 (Ser-176) (lanes 5 & 6), and anti-EB3 (a.a. 171-182) (lanes 7 & 8).

Immunocytochemical labeling of EB3 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-α-Tubulin (TM4111) (green) and rat monoclonal anti-EB3 (EM5091) (red). The antibodies were detected using either goat anti-mouse:DyLight® 488 (MS3011) or goat anti-Rat:DyLight® 594 (RS3111).



Rat monoclonal antibodies are supplied in phosphate-buffered saline and 0.05% sodium azide. Rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

EM5101 Komarova, Y. et al. (2005) Mol Biol Cell. 16(11):5334. WB, ICC: CHO-K1 cellls

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
EB3 Rat mAb50 μlWB, E, ICCHu, Rt, Ms32
EB3 Rat mAb50 μlWB, E, ICCHu, Rt, Ms32
EB3 (a.a. 171-182) Rabbit pAb50 μlWB, EHu, Rt, Ms32
EB3 (Ser-162), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms32
EB3 (Ser-176), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms32