Estrogen receptor α (ERα) is a member of the steroid receptor superfamily and its structure includes an N-terminal ligand-independent transactivation domain (AF-1), a highly conserved DNA binding domain, and a C-terminal ligand-dependent transactivation domain (AF-2). AF-1 and AF-2 activate transcription independently and synergistically, and act in a promoter- and cell-specific manner. Phosphorylation at multiple sites provides an important mechanism to regulate ERα activity. Ser-104, Ser-106, Ser-118, and Ser-167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERα activity. In addition to these sites, phosphorylation of Tyr-537 has been implicated in maximal hormone binding, dimerization, and transcriptional activity. Tyr-537, located in the AF-2 domain, is phosphorylated by c-Src leading to nuclear export of ERα and degradation. Thus, a variety of phosphorylation events control ERα activity.
Castoria, G. et al. (2012) Oncogene. 31:4868.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
This kit contains:
|CATALOG#||DESCRIPTION||SIZE||APPLICATIONS||SPECIES REACTIVITY||MW (kDa)|
|Estrogen Receptor α(C-terminus) Rabbit pAb||50 μl||WB, E, IP, ICC||Hu, Rt, Ms||68|
|Estrogen Receptor α (Tyr-537), phospho-specific Rabbit pAb||50 μl||WB, E||Hu, Rt, Ms, Ck, Fr||68|
|Estrogen Receptor α (Tyr-537), phospho-specific Mouse mAb||50 μl||WB, E||Hu, Rt, Ms, Ck, Fr||68|
|Anti-Mouse Ig:HRPDonkey pAb||100 μl||WB, E||Ms|
|Anti-Rabbit Ig Light ChainSpecific:HRP Mouse mAb||100 μl||WB, E, ICC, IHC||Rb|
The ERα Phospho-Regulation antibody sampler kit can be used to detect phosphorylation of Tyr-537 relative to total ERα expression levels. The kit includes mouse monoclonal and rabbit polyclonal antibodies to detect phospho-Tyr-537, as well as a rabbit polyclonal antibody to detect ERα. The kit also includes anti-Rabbit Light Chain specific:HRP and anti-Mouse Ig specific:HRP secondary reagents for detection of antibodies in Western blot, ELISA, or immunocytochemistry.